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| 大川芎方效应组分血中移行成分UPLC-MS分析 | |
| 引用本文： | 魏元锋,林晓,张宁,冯怡.大川芎方效应组分血中移行成分UPLC-MS分析[J].中国中药杂志,2011,36(9):1245-1248. |
| 作者姓名： | 魏元锋 林晓 张宁 冯怡 |
| 作者单位： | 1. 中国药科大学,中药学院,江苏,南京,211198 2. 上海中医药大学,中药学院,上海,201203上海中医药大学,中药学院,上海,201203 3. 上海中医药大学,中药现代制剂技术教育部工程研究中心,上海,201203 |
| 基金项目： | 上海市教育委员会科研创新项目(09YZ117);上海市教育委员会重点学科项目(J50302) |
| 摘 要： | 目的:研究硝酸甘油致实验性偏头痛模型大鼠灌胃大川芎方效应组分后血中移行成分及其归属.方法:以UP-LC-MS作为分析手段,以大川芎方效应组分、川芎效应组分、天麻效应组分、天麻素、阿魏酸、洋川芎内酯Ⅰ作为对照,综合分析总离子流色谱图、质量色谱图及质谱图等信息,通过对比各色谱峰的保留时间及质谱图特征,确认硝酸甘油致实验性偏头痛模型大鼠灌胃大川芎方效应组分后血中移行成分及其归属.结果:大川芎方效应组分给药后血浆中共发现10个人血成分,其中4个为天麻素等天麻效应组分中原型成分,6个为阿魏酸、洋川芎内酯Ⅰ等川芎效应组分中原型成分.结论:本研究将为阐明大川芎方效应组分药效物质基础提供依据. |
| 关 键 词： | 大川芎方 效应组分 UPL-MS 移血成分 |
| 收稿时间： | 2010/9/21 0:00:00 |
UPLC-MS analysis of constituents of Dachuanxiong Fang active parts absorbed into blood |
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| WEI Yuanfeng,LIN Xiao,ZHANG Ning and FENG Yi.UPLC-MS analysis of constituents of Dachuanxiong Fang active parts absorbed into blood[J].China Journal of Chinese Materia Medica,2011,36(9):1245-1248. | |
| Authors: | WEI Yuanfeng LIN Xiao ZHANG Ning and FENG Yi |
| Institution: | College of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 211198, China;College of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicinal, Shanghai 201203, China;Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicinal of Ministry of Education , Shanghai University of Traditional Chinese Medicinal, Shanghai 201203, China;Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicinal, Shanghai 201203, China;Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicinal of Ministry of Education , Shanghai University of Traditional Chinese Medicinal, Shanghai 201203, China |
| Abstract: | Objective : To study the constituents absorbed into blood following the intragstric administration of Dachuanxiong Fang active parts(DCXF) to the experimental migrainous model rats induced by nitroglycerin. Method : The UPLC-MS was used as the analytic method. The DCXF, Chuanxiong active parts, Tianma active parts, gastrodin, ferulic acid and senkyunolide I were used as the control samples. The information on the total ion chromatogram, mass chromatogram and the mass spectrogram were synthetically analyzed to confirm the constituents absorbed into blood. Result : Ten of the DCXF constituents were detected in the rats plasma post the intragastric administration of DCXF , in whitch four including gastrodin came from Tianma active parts and six including ferulic acid and senkyunolide I from Chuanxiong active parts. Conclusion : The findings abtained from the study can provide the useful information for the determination of bioactive substances of the DCXF. |
| Keywords: | DCXF active parts UPLC-MS constituents absorbed into blood |
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