不同宫颈组织eIF-4A蛋白表达及其临床意义探讨

目的：探讨真核细胞起始因子eIF-4A在正常及不同级别病变宫颈组织中的表达及其临床意义。方法：选取青岛大学医学院附属医院妇科2011-01-0l-2012-06-01行手术治疗或活检确诊的120例患者，其中低度鳞状上皮内病变组织（CINI级）30例，高度鳞状上皮内病变组织（CINⅡ和Ⅲ级）30例及宫颈癌组织60例（51例鳞癌，9例非鳞癌）。另选取同期手术切除的30例正常宫颈组织作为对照。应用免疫组织化学方法测定不同宫颈组织中eIF-4A蛋白的表达水平，探讨其与宫颈癌临床病理参数之间的关系。结果：eIF_4A蛋白的阳性表达率在正常宫颈组织（0）、低度鳞状上皮内病变（46．7％）、高度鳞状上皮内病变（76．7％）及宫颈鳞状细胞癌组织（78．4％）中逐渐增高，差异有统计学意义，x2=54．421，P〈0．001；且趋势检验有统计学意义，x2=47．111，P〈0．001；但是高度鳞状上皮内病变组织和宫颈鳞状细胞癌组织中elF-4A的阳性表达差异无统计学意义，x2=0．034，P=0．854。eIF-4A蛋白表达与宫颈癌组织学类型无关，x2=0．592，P=0．442；与宫颈癌的病理分级有关，x2=6．161，P=0．046；与临床分期有关，x2=7．515，P-0．006。结论：eIF-4A可能参与了宫颈病变发生和发展的过程，可能在宫颈病变的初始过程即发挥了作用。

Expression of eIF-4A in cervical tissue and its clinical significance

OBJECTIVE： To explore the expression of elF-4A in cervical tissue and its clinical significance. METH- ODS： Pathologically confirmed cervical specimens,including 30 cases of CIN I , 30 cases of CIN II and Ⅲ , 60 cases of cervical intraepithelial cancer,and 30 normal cervical tissues,were selected from 2011-01-01 to 2012-06-01 in Department of Gynecology, Affiliated Hospital of Medical College of Qingdao University. Immunohistochemistry was used to detect the expression of eIF-4A in differential cervical tissues to explore its relationship with clinicopathological parameters between cervical cancers. RESULTS： The expression of elF-4A was not detected in normal cervical tissue. The expression level was gradually increased with increment of grading of epithelial lesions in the order of CIN I （46. 7%）, CIN I andⅢ（76.7%） and SCC（78.4%）. The difference among the four groups was statistically significant （（2 = 54. 421 ,P〈0. 001） and the trend test was statistically significant too （X2 = 47. 111, P〉0. 001）, hut there was no significant difference be tween high-grade squamous intraepithelial lesions and cervical squamous cell carcinoma （X2 = 0. 034, P = 0. 854）. The overexpression of eIF-4A was significantly related to the differentiation and clinical stage of tumor （x2= 6. 161, P= 0. 046;）C = 7. 515, P = 0. 006）, but it was not significant correlated with the histopathological types （x2= 0. 592, P= 0. 442）. CONCLUSION： The overexpression of eIF-4A in cervical cancer may play a role in the oncogenesis and malignant progression of cervical cancer.