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| 环介导等温扩增技术及其在病原微生物检测中的应用 | |
| 引用本文： | 肖维威,周琳华,郑文岭,马文丽. 环介导等温扩增技术及其在病原微生物检测中的应用[J]. 基础医学与临床, 2013, 33(1): 19-13 |
| 作者姓名： | 肖维威 周琳华 郑文岭 马文丽 |
| 作者单位： | 南方医科大学基因工程研究所,广东广州,510515 |
| 摘 要： | 环介导等温扩增(LAMP)技术是近年发展起来的一项新的快速核酸恒温扩增技术，具有高效、快速、特异、易检测、易操作等特点，自面世以来被科学家认为是能替代常规PCR的一项扩增技术，非常适用于现场检测和基层检测。本文就LAMP技术的原理、特点、进展及其在病原微生物检测中的应用进行了简要的概述。 |
Loop-mediated isothermal amplification technology and its application in pathogenic microorganism detection |
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| XIAO Wei-wei,ZHOU Lin-hua,ZHENG Wen-ling,MA Wen-li. Loop-mediated isothermal amplification technology and its application in pathogenic microorganism detection[J]. Basic Medical Sciences and Clinics, 2013, 33(1): 19-13 | |
| Authors: | XIAO Wei-wei ZHOU Lin-hua ZHENG Wen-ling MA Wen-li |
| Affiliation: | *(Institute of Gene Engineering,Southern Medical University,Guangzhou 510515,China) |
| Abstract: | Loop-mediated isothermal amplification (LAMP) technology is a novel rapid nucleic acid isothermal amplification technology developed in recent years. Because of its high effiency, rapidity, specificity, and simplicity, scientists believe it will be a common amplification technology replacing the conventional PCR method. This paper briefly reviews the principle of the technology, techinical characteristics, progress, and its application in pathogenic microorganism detection. |
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