L-精氨酸对慢性阻塞性肺病大鼠模型的肺功能保护

目的探讨L-精氨酸对被动吸烟致肺损伤大鼠的肺功能保护作用。方法采用香烟熏吸加气道内注入脂多糖法建立慢性阻塞性肺病（COPD）大鼠模型，并应用L-精氨酸干预，检测各组大鼠的肺功能、动脉血气，检测肺组织匀浆的NO及一氧化氮合酶（NOS）含量，观察肺组织病理学改变并进行形态学定量检测，检测肺组织内皮型NOS（eNOS）和诱生型NOS（iNOS）的表达情况。结果大鼠模型基本符合人类COPD组织病理学改变特点，应用L-精氨酸干预后，大鼠的肺组织损伤减轻，肺功能改善。结论应用L-精氨酸干预，对于COPD的形成有预防保护作用。

Protective effects of L-arginine on the lung function of rats with chronic obstructive pulmonary disease

[Objective] To investigate the protective and therapeutic effects of L-Arginine on the pathogenesis of chronic obstructive pulmonary disease(COPD) in rats.[Methods] COPD animal model was established by smoke inhalations and intratracheal instillations of lipopolysaccharide in Wistar rats,and received LArginine treatment daily transesophageally.Lung function test,blood gas analysis,light microscopy and electromicroscopy observations were performed in each group.The concentration of NO and NOS in lung tissue homogenate were measured.The expression of eNOS and iNOS were observed.[Results] Smoking for 1 months and instillations of lipopolysaccharide twice resulted in chronic bronchitis and emphysema.Administration of L-arginine resulted in statistically significant inhibition of pulmonary injury and the lung function was improved.[Conclusion] L-argnine may have a protective effect on COPD model of rats.