RhD阴性献血者RhD弱抗原变异体的发生频率

目的了解RhD阴性献血者RhD弱抗原变异体的发生频率,为提高输血安全性提供理论依据。方法首先采用盐水试管法对初筛为RhD阴性的血样进行RhD表型鉴定;然后采用间接抗球蛋白试验筛查Partial D和Weak D,最后用吸收放散试验从间接抗球蛋白试验确认阴性的血样中筛选并确认DEL型。结果 RhD阴性献血者中最多的Rh表型为dccee,其次为dCcee,分别为57.34%（207/361）和32.13%（116/361）。共筛查出Partial D和Weak D 19例,总检出率为5.26%（19/361）,其中dccEe 8例（2.22%）、dCcee 5例（1.39%）、dCCee 4例（1.11%）、dccee和dCcEe各1例（0.28%）。共确认DEL型87例,总检出率为24.10%（87/361）,其中dCcee 72例（19.94%）、dCCee 8例（2.22%）和dccEe 7例（1.94%）。结论 RhD阴性献血者RhD弱抗原变异体发生频率较高。常规血清学检测Rh阴性的供血者,应进一步排除RhD弱抗原变异体,以保障临床输血安全。

Frequency of RhD weak antigen variant in RhD negative blood donors

Objective To understand the frequency of RhD weak antigen variant in RhD negative blood donors,as to provide theoretical basis for improve transfusion safety.Methods First,salt water test tube method was used for screening for RhD negative blood phenotype identification;then indirect antiglobulin test（IAT）screening Partial D and Weak D;the DEL was confirmed with adsorption and elusion finally.Results The most Rhphenotype was the dccee in RhD negative blood donors,followed by dCcee,57.34%（207/361）and 32.13%（116/361）,respectively.19cases of Partial D and Weak D were screening out,the overall detection rate was 5.26%（19/361）,including the dccEe 8cases（2.22%）,5cases（1.39%）,dccEe 4cases（1.11%）,dccEe and dccEe each in 1case（0.28%）.87cases of DEL type were confirmed with total detection rate of 24.10%（87/361）,including the dCcee 72 cases（19.94%）,8cases（2.22%）and dCcee 7cases（1.94%）.Conclusion Frequency of RhD weak antigen variant in RhD negative blood donors was higher.It would be of important value that further research and clarify the nature of RhD weak antigen variants.