黄芪建中汤对脾胃虚寒证胃溃疡大鼠的影响

目的探讨黄芪建中汤对脾胃虚寒证胃溃疡大鼠的影响。方法将SD大鼠随机分为正常组、模型组、埃索美拉唑组(4.17 mg/kg)及黄芪建中汤低、高剂量组(9.27、18.54 g/kg)。采用番泻叶(10 m L/kg)联合游泳力竭法建立脾胃虚寒证候模型,无水乙醇(10 m L/kg)联合阿司匹林肠溶片(200 mg/kg)建立胃溃疡模型。观察并记录大鼠的整体状态、体质量、进食量、饮水量和肛温,肉眼及HE染色观察胃黏膜组织形态学损伤,测量胃液总酸度和胃蛋白酶活性,ELISA法检测大鼠血清IL-4、IL-10、NO和TNF-α水平,qRT-PCR和免疫组化法分别检测TLR-2、MyD88 mRNA及蛋白表达。结果与模型组比较,黄芪建中汤组大鼠体质量、进食量、饮水量和肛温均上升(P<0.05,P<0.01),溃疡指数下降(P<0.01),胃酸总酸度和胃蛋白酶活性均降低(P<0.01),血清TNF-α水平下降(P<0.05、P<0.01),IL-4、IL-10和NO水平上升(P<0.05、P<0.01),胃组织中TLR-2、MyD88 mRNA及蛋白表达下调(P<0.01)。结论黄芪建中汤对脾胃虚寒型胃溃疡大鼠有显著的疗效,其作用机制可能通过调节TLR-2/MyD88信号通路,影响炎性因子表达,下调黏膜攻击因子水平,从而加速胃黏膜溃疡修复。

Effects of Huangqi Jianzhong Decoction on rats with gastric ulcer of SpleenStomach Deficiency and Cold Pattern

AIM To investigate the effects of Huangqi Jianzhong Decoction( HQJZD) on rats with gastric ulcer( GU) due to Spleen-Stomach Deficiency and Cold( SSDC). METHODS SD rats were randomly divided into normal group,model group,esomeprazole group( 4. 17 mg/kg) and low dose and high dose HQJZD groups(9. 27,18. 54 g/kg). The SSDC rat models were established by senna administration(10 m L/kg) and swimming exhaustion method;subsequently,GU models were induced by anhydrous ethanol(10 m L/kg) and aspirin entericcoated tablets(200 mg/kg). The rats had their overall state,body weight,food intake,water intake and anal temperature observed and recorded;their histomorphological damage of gastric mucosa observed by naked eye and HE staining;their serum levels of IL-4,IL-10,NO and TNF-α detected by ELISA method;and their TLR-2,MyD88 mRNA and protein expression detected by qRT-PCR and immunohistochemical methods,respectively. RESULTS Compared with the model group,HQJZD groups shared increased body weight,food intake,water intake,and anal temperature( P < 0. 05,P < 0. 01);decreased total acidity of gastric juice and pepsin activity( P<0. 01);decreased serum TNF-α levels( P < 0. 05,P < 0. 01),and increased IL-4,IL-10 and NO levels( P < 0. 05,P <0. 01);and down-regulated TLR-2, MyD88 mRNA and protein expression in gastric tissues( P < 0. 01).CONCLUSION HQJZD is significantly effective to GU rats due to SSDC,and its mechanism may be related to regulation of the TLR-2/MyD88 signaling pathway,to changes of the inflammatory factors,and to down-regulation of the mucosal attack factors levels. Taken together,the changes beneficially affect gastric mucosal ulcer.