小构树总黄酮提取工艺优化及其抗氧化、美白活性

目的优化小构树总黄酮提取工艺,并评价其抗氧化、美白活性。方法在单因素试验基础上,以乙醇体积分数、液料比、提取温度、提取时间为影响因素,总黄酮得率为评价指标,Box-Behnken响应面法优化提取工艺。检测总黄酮对DPPH、ABTS自由基的清除能力,以及对酪氨酸酶活性的抑制作用。结果最佳条件为乙醇体积分数90%,液料比35∶1,提取温度85℃,提取时间80 min,总黄酮得率为55.14 mg/g。总黄酮对DPPH、ABTS自由基及酪氨酸酶单酚酶、二酚酶的IC50值分别为151.70、242.20、24.37、15.64μg/m L。结论该方法稳定可靠,可用于提取具有良好抗氧化、美白活性的小构树总黄酮。

Extraction process optimization and anti-oxidant,whitening activities of total flavonoids from Broussonetia kazinoki

AIM To optimize the extraction process for total flavonoids from Broussonetia kazinoki Sieb.et Zucc.and to evaluate their anti-oxidant and whitening activities.METHODS With ethanol concentration,liquidsolid ratio,extraction temperature and extraction time as influencing factors,total flavonoids yield as an evaluation index,the extraction process was optimized by Box-Behnken response surface method on the basis of single factor test.The scavenging capacities of total flavonoids on DPPH and ABTS free radicals,and their inhibitory effect on tyrosinase activity were detected.RESULTS The optimal conditions were determined to be 90%for ethanol concentration,35∶1 for liquid-solid ratio,85℃for extraction temperature and 80 min for extraction time,the total flavonoids yield was 55.14 mg/g.The IC50values of total flavonoids on DPPH and ABTS free radicals,tyrosinase monophenolase and diphenolase were 151.70,242.20,24.37,15.64μg/m L.CONCLUSION This stable and reliable method can be used for the extraction for total flavonoids from B.kazinoki with good anti-oxidant and whitening activities.