三益丹对大鼠心肌缺血的改善作用

目的观察三益丹对大鼠心肌缺血的改善作用。方法皮下多点注射ISO(85 mg/kg)构建SD大鼠心肌缺血模型,随机分为正常组,模型组(ISO 85 mg/kg),复方丹参滴丸组(85 mg/kg)、三益丹低、中、高剂量组(37.5、75.0、150.0 mg/kg),连续灌胃给药14 d。心电图监测大鼠心电图ST段的变化;HE染色观察心肌病理损伤程度;检测大鼠血清CK、LDH、AST心肌酶水平,cTn-I、MDA、NO、TNF-α、IL-6水平。结果与模型组比较,三益丹抑制心肌缺血大鼠心电图ST段抬高(P<0.05),降低血清中CK、LDH、AST水平(P<0.05),降低cTn-I、MDA、NO、TNF-α、IL-6水平(P<0.05),减轻心肌病理损伤程度。结论三益丹对ISO诱导大鼠心肌缺血模型具有明显的改善作用。

Effects of Sanyidan on myocardial ischemia in rats

AIM To observe the effects of Sanyidan on myocardial ischemia in rats.METHODS Multiple subcutaneous injections of isproterenol(ISO, 85 mg/kg) were practiced upon SD rats to construct myocardial ischemia models. The rats were randomly divided into normal group, model group(ISO,85 mg/kg), and compound Danshen dripping pills group(85 mg/kg), low-dose, medium-dose and high-dose Sanyidan groups(37.5, 75.0, 150.0 mg/kg) for 14-day intragastric administration. The rats had their changes in ST segment monitored by electrocardiogram, their degree of myocardial pathological damage observed by HE staining, and their serum levels of CK, LDH, AST myocardial enzymes, and cTn-I, MDA, NO, TNF-α, and IL-6 detected as well.RESULTS Compared with the model group, myocardial ischemic rats of Sanyidan groups shared inhibited ST-segment elevation(P<0.05), reduced serum CK, LDH, AST levels(P<0.05), and decreased cTn-I, MDA, NO, TNF-α, IL-6 levels(P<0.05), and attenuated degree of myocardial pathological damage as well.CONCLUSION Sanyidan is significantly effective in improving myocardial ischemia of ISO-induced rat models.