| HPLC法同时测定筋骨伤喷雾剂中7种成分 |
| |
| 引用本文： | 李佳蔚,王娇,徐家怡,程吉祥,刘青,伍庆. HPLC法同时测定筋骨伤喷雾剂中7种成分[J]. 中成药, 2020, 0(2): 301-304 |
| |
| 作者姓名： | 李佳蔚 王娇 徐家怡 程吉祥 刘青 伍庆 |
| |
| 作者单位： | 1.贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室;2.贵州远程制药有限责任公司 |
| |
| 基金项目： | 国家新药创制重大专项-中药经典名方开发子课题(2015ZX09101043-007);国家中药标准化项目(ZYBZH-C-GZ-10);喀斯特地区生物与信息技术协同创新中心(黔教合协同创新字[2014]04);贵州中药生产过程质量控制研究(黔教合KY字[2018]014) |
| |
| 摘 要： | 目的建立HPLC法同时测定筋骨伤喷雾剂(赤胫散、赤芍、淫羊藿等)中芍药苷、鞣花酸、朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷Ⅰ的含有量。方法该药物水饱和正丁醇提取液的分析采用Agilent C18色谱柱(4.6 mm×150 mm,5μm);流动相乙腈-0.05 mol/L磷酸二氢钾-甲醇,梯度洗脱;体积流量1.0 mL/min;柱温35℃;检测波长230、254、270 nm。结果 7种成分在各自范围内线性关系良好(r≥0.999 5),平均加样回收率93.5%～99.6%,RSD 1.41%～2.86%。结论该方法准确可靠,重复性好,可用于筋骨伤喷雾剂的质量控制。
|
| 关 键 词： | 筋骨伤喷雾剂 化学成分 HPLC |
Simultaneous determination of seven constituents in Jingushang Spray by HPLC |
| |
| LI Jia⁃yu,WANG Jiao,XU Jia⁃yi,CHENG Ji⁃xiang,LIU Qing,WU Qing. Simultaneous determination of seven constituents in Jingushang Spray by HPLC[J]. Chinese Traditional Patent Medicine, 2020, 0(2): 301-304 |
| |
| Authors: | LI Jia⁃yu WANG Jiao XU Jia⁃yi CHENG Ji⁃xiang LIU Qing WU Qing |
| |
| Affiliation: | (Guizhou Provincial Key Laboratory for Information System and Protection of Mountainous Environment,Guizhou Normal University,Guiyang 550001,China;Guizhou Yuancheng Pharmaceutical Co.,Ltd.,Guiyang 550018,China) |
| |
| Abstract: | AIM To establish an HPLC method for the simultaneous content determination of paeoniflorin,ellagic acid,epimedin A,epimedin B,epimedin C,icariin and baohuosideⅠin Jingushang Spray(Polygonum runcinatum Buch.⁃Ham.ex D.Don var.sinense Hemsl.,Paeoniaeradix Rubra,Epimedii Folium,etc.).METHODS The analysis of water⁃saturated n⁃butanol extract of this drug was performed on a 35℃thermostatic Agilent C18 column(4.6 mm×150 mm,5μm),with the mobile phase comprising of acetonitrile-0.05 mol/L po⁃tassium dihydrogen phosphate⁃methanol flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 230,254,270 nm.RESULTS Seven constituents showed good linear relationships within their own ranges(r≥0.9995),whose average recoveries were 93.5%-99.6%with the RSDs of 1.41%-2.86%.CONCLUSION This accurate,reliable and reproducible method can be used for the quality control of Jingushang Spray. |
| |
| Keywords: | Jingushang Spray chemical constituents HPLC |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
