| HPLC法同时测定十三味和中丸中9种成分 |
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| 引用本文： | 徐倩,杨满琴,鲍学梅,葛丹丹,徐国兵,胡云飞,谢若男.HPLC法同时测定十三味和中丸中9种成分[J].中成药,2020(2):297-300. |
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| 作者姓名： | 徐倩 杨满琴 鲍学梅 葛丹丹 徐国兵 胡云飞 谢若男 |
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| 作者单位： | ;1.安徽中医药大学第二附属医院;2.安徽省食品药品检验研究院;3.安徽中医药大学;4.亳州学院 |
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| 基金项目： | 国家质量监督检验检疫总局科技计划项目(2015QK098);安徽中医药大学校级自然科学基金项目(2017fyyb009) |
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| 摘 要： | 目的建立HPLC法同时测定十三味和中丸(柴胡、枳壳、延胡索等)中芍药苷、延胡索乙素、盐酸小檗碱、柚皮苷、橙皮苷、黄芩苷、甘草酸、柴胡皂苷a、柴胡皂苷d的含有量。方法该药物70%甲醇提取液的分析采用Waters SunfireTMC18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈?0.2%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长210、230、283、345 nm。结果9种成分在各自范围内线性关系良好(R2≥0.9991),平均加样回收率95.1%~102.3%,RSD 0.48%~3.11%。结论该方法简便可靠,可用于十三味和中丸的质量控制。
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| 关 键 词： | 十三味和中丸 化学成分 HPLC |
Simultaneous determination of nine constituents in Shisanwei Hezhong Pills by HPLC |
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| Institution: | (The Second Hospital Affiliated to Anhui University of Chinese Medicine,Hefei 230061,China;Anhui Provincial Institute for Food and Drug Control,Hefei 230051,China;Anhui University of Chinese Medicine,Hefei 230031,China;Bozhou University,Bozhou 236800,China) |
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| Abstract: | AIM To establish an HPLC method for the simultaneous content determination of paeoniflorin,tet?rahydropalmatine,berberine hydrochloride,naringin,hesperidin,baicalin,glycyrrhizic acid,saikosaponin a and saikosaponin d in Shisanwei Hezhong Pills(Bupleuri Radix,Aurantii Fructus,Corydalis Rhizoma,etc.).METHODS The analysis of 70%methanol extract of this drug was performed on a 30℃thermostatic Waters SunfireTM C18 column(4.6 mm×250 mm,5μm),with the mobile phase comprising of acetonitrile?0.2%phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 210,230,283,345 nm.RESULTS Nine constituents showed good linear relationships within their own ranges(R2≥0.9991),whose average recoveries were 95.1%-102.3%with the RSDs of 0.48%-3.11%.CONCLUSION This simple and reliable method can be used for the quality control of Shisanwei Hezhong Pills. |
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| Keywords: | Shisanwei Hezhong Pills chemical constituents HPLC |
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