一测多评法同时测定宽中顺气丸中8种成分

目的建立一测多评法同时测定宽中顺气丸(香附、陈皮、莪术等)中橙皮苷、川陈皮素、桔皮素、莪术二酮、莪术醇、吉马酮、香附烯酮、α-香附酮的含有量。方法该药物甲醇提取液的分析采用Agilent TC-C18色谱柱(250 mm×4. 6 mm,5μm);流动相乙腈-0. 1%冰醋酸,梯度洗脱;体积流量1. 0 m L/min;检测波长214、242、326 nm;柱温25℃。以吉马酮为内标,建立其他7种成分相对校正因子,测定含有量。结果 8种成分在各自范围内线性关系良好(r≥0. 999 2),平均加样回收率为96. 93%~100. 00%,RSD为0. 81%~1. 63%。一测多评法所得结果与外标法接近。结论该方法简便准确,可用于宽中顺气丸的质量控制。

Simultaneous determination of eight constituents in Kuanzhong Shunqi Pills by QAMS

AIM To establish a quantitative analysis of multi-components by single-marker(QAMS)method for the simultaneous content determination of hesperidin,nobiletin,tangeretin,curdione,curcumol,germacrone,cyperotundone andα-cyperone in Kuanzhong Shunqi Pills(Cyperi Rhizoma,Citri reticulatae Pericarpium,Curcumae Rhizoma,etc.).METHODS The analysis of methanol extract of this drug was performed on a 25℃thermostatic Agilent TC-C18column(250 mm×4.6 mm,5μm),with the mobile phase comprising of acetonitrile-0.1%glacial acetic acid flowing at 1.0 m L/min in a gradient elution manner,and the detection wavelengths were set at 214,242,326 nm.Germacrone was used as an internal standard to calculate the relative correction factors of the other seven constituents,after which the content determination was made.RESULTS Eight constituents showed good linear relationships within their own ranges(r≥0.999 2),whose average recoveries were 96.93%-100.00%with the RSDs of 0.81%-1.63%.The results obtained by QAMS approximated those obtained by external standard method.CONCLUSION This simple and accurate method can be used for the quality control of Kuanzhong Shunqi Pills.