Establishment of Biomimetic Soft Tissue Integration with the Surface of Zirconia Fused with Platelet-Activating Peptide |
| |
| Authors: | Chia-Yu Chen Wonwoo Jang David M. Kim Masazumi Nagai Shigemi Nagai |
| |
| Affiliation: | Department of Oral Medicine, Immunity and Infection, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA 02115, USA; (W.J.); (D.M.K.); (M.N.); (S.N.) |
| |
| Abstract: | ![]()
Soft tissue sealing around zirconia (ZrO2) abutment is critical for the long-term stability of dental implants. The goal of the study is to develop a strong basal lamina (BL)-mediated epithelial attachment to ZrO2 via a novel physicochemical immobilization method. An electrophoretic fusion (EPF) method was applied to fuse a phosphonic acid (PA) linker to ZrO2 discs. Bindings of the PA linker and the following protease activated receptor 4 (PAR4) were verified by Fourier-transform infrared spectroscopy (FITR). Then, ZrO2 discs were doped in platelet-rich plasma (PRP). Platelet-derived growth factor (PDGF) was measured to assess platelet activation. PRP-doped discs were subsequently co-cultured with human gingival epithelial cells (OBA9) to evaluate establishment of basal lamina-mediated epithelial attachment. The EPF method achieved robust immobilization of the PA linker and PAR4 onto the ZrO2 surface. The resultant PAR4-coupled ZrO2 successfully induced platelet aggregation and activation. Furthermore, a BL-mediated epithelial attachment was established. The results are significant for clinical application to minimize the risk of developing peri-implant diseases. |
| |
| Keywords: | implant surface zirconia epithelial attachment soft tissue sealing |
|
|
