RANKL浓度对破骨细胞形成与活性的影响

目的 探讨不同浓度RANKL对骨髓单核细胞向破骨细胞分化和活性的影响.方法 50、100和150 μg/L三种RANKL浓度诱导骨髓单核细胞向破骨细胞分化.抗酒石酸酸性磷酸酶染色观察破骨细胞形态并计数,抗酒石酸酸性磷酸酶活性检测试剂盒检测上清中的抗酒石酸酸性磷酸酶活性,骨板吸收实验检测破骨细胞骨吸收活力.结果 100 μg/L和150 μg/L RANKL浓度组在破骨细胞数量、细胞大小、核数目,抗酒石酸酸性磷酸酶活性和骨吸收率方面均明显高于50 μg/L组（P＆lt;0.01）,而100 μg/L和150 μg/L组间没有明显差异.结论 100 μg/L RANKL浓度下破骨细胞形成和活性能力强且相对较经济,是破骨细胞诱导培养的理想浓度值.

Effect of RANKL on formation and activity of osteoclasts.

Objective To study the effects of RANKL on the formation and activity of osteoclasts. Methods RANKL at concentrations of 50, 100 and 150μg/L induced osteoclastogenesis from bone marrow mononuclear cells. Tartrate resistant acid phosphatase staining was used to observe the formation and morphology of osteo clasts. Tartrate resistant acid phosphatase assay kit was used to evaluate the tartrate resistant acid phosphatase ac- tivity in the culture supernatant. Bone absorption ability of osteoclats was evaluated by absorption assay of bone plate. Results The number, sizes, nucleus number, tartrate resistant acid phosphatase activity and bone absorp- tion ability of osteoclasts were enhanced in 100 μg/L and 150 μg/L RANKL groups as compared with 50μg/L RANKL group （P〈0. 01 ）. There was no significant difference between 100μg/L and 150 μg/L RANKL groups. Conclusion 100μg/L RANKL can enhance formation and activity of osteoclasts, and is an ideal concentration for osteoclast culture.