丹参酮衍生物对K562细胞的生长抑制及诱导凋亡作用研究

目的研究丹参酮衍生物对人红白血病细胞株K562的生长抑制以及诱导凋亡作用。方法MTT比色法检测不同浓度丹参酮1、丹参酮2和丹参酮B对K562细胞的增殖抑制作用;PI单染法检测3者对K562细胞周期的影响;An-nexin V/PI双染法检测3者对K562细胞诱导凋亡的作用。结果丹参酮1和丹参酮B能够抑制K562细胞增殖,IC50分别为5.22和15.11μmol·L-1,且分别在2.5~10μmol·L-1和10~40μmol·L-1剂量范围内,G0/G1期细胞比例的增加及早期凋亡细胞百分率的提高均呈剂量相关性;丹参酮2在100μmol·L-1的剂量下,对K562细胞的抑制率仅为27.8%,无诱导其凋亡作用,但可以使G0/G1期细胞增多。结论丹参酮1和丹参酮B抑制K562细胞增殖,阻滞其于G0/G1期并诱导细胞凋亡;丹参酮2对K562细胞没有明显生长抑制及诱导凋亡作用,但可将其阻滞于G0/G1期。

Study on the effects of tanshinone derivates on growth inhibition and apoptosis induction in human erythroleukemia cell line K562

Aim To investigate the effects of tanshinone derivates on growth inhibition and apoptosis induction in human erythroleukemia cell line K562.Methods MTT assay was used to measure the inhibition effect on the proliferation of K562 cells cultured with tanshinone 1,tanshinone 2 and tanshinone B at different concentrations.The effect of test compounds on cell cycle in K562 was detected by PI staining.The apoptosis induction effect was measured by Annexin V/PI double staining.Results Tanshinone 1 and tanshinone B could inhibit the proliferation of K562 cells,the 50% inhibiting concentrations of them were 5.22 and 15.11 μmol·L-1 respectively,during the dose of 2.5～10 μmol·L-1 and 10～40 μmol·L-1,the proportion of G0/G1 phase cells increased,and the percentage of the cells in early phase of apoptosis raised as the concentration of drugs increased;the inhibition rate of K562 cells treated with 100 μmol·L-1 tanshinone 2 was just 27.8%,tanshinone 2 could not induce apoptosis in K562 cells,but could increase the number of cells in the G0/G1 phase.Conclusions Tanshinone 1 and tanshinone B could inhibit the proliferation of K562 cells,arrest them in the G0/G1 phase,and induce apoptosis.Tanshinone 2 had no obvious toxic and apoptosis induction effect on K562 cells,but it could arrest them in the G0/G1 phase.