阿托伐他汀对oxLDL诱导泡沫细胞Lkn-1表达的影响

目的观察阿托伐他汀对OX-LDL诱导U937细胞形成泡沫细胞过程中Lkn-1表达的影响，探讨阿托伐他汀抗AS的作用机制。方法在由O．1μmol／L佛波脂（PMA）诱导分化人U937巨噬细胞中加入100mg／Lox—LDL及不同浓度（0．1、1、10μmol／L）的阿托伐他汀共同孵育24h，分别用酶联免疫吸附试验（ELISA）和RT-PCR方法检测培养细胞上清中Lkn-1的表达变化。结果OX-LDL组较正常对照组Lkn-1的表达明显增加（P〈0．05）。给药各组较OX-LDL组Lkn-1明显减少（P〈0．05）。且随阿托伐他汀浓度的增加Lkn-1表达呈逐渐减少的趋势（P〈0．05）。结论阿托伐他汀能抑制ox—LDL诱导U937细胞系形成泡沫细胞过程炎症因子Lkn-1的表达和分泌，可能为其抗动脉粥样硬化的重要机制之一。

The effect of atorvastatin on the expression of Lkn-1 during U937 monocyte differentiating into foam cells induced by oxidized LDL

Objective To observe the effect of atorvastatin （AT） on the expression of Lkn-1 during U937 monocyte differentiating into foam cells induced by oxidized LDL （ox-LDL） ,and to explore the antiatherosclerotic action mechanism of AT. Methods The macrophages derived from U937 monocytes were induced by PMA （0.1μmoL/L） , which were treated with 100mg/L ox-LDL plus different concentrations of AT （0.1,1,10μmol/L） for 24 hours. Then the expression levels of Lkn-1 in supernatant of cultured cell were detected by ELISA and RT-PCR. Results As compared with those in control group, the expression levels of Lkn-1 of in ox-LDL group were significantly increased （ P 〈 0.05 ）. However the expression levels of Lkn-1 in different concentrations of AT groups were significantly decreased, as compared with those in ox-LDL group （ P 〈 0.05 ）. Moreover with the increase of AT concentration, the the expression levels of Lkn-1 were gradually decreased （ P 〈 0. 05 ）. Conclusion Atorvastatin can inhibit the expression and secretion of Lkn-1 in the course of U937 monocyte differentiating into foam cells induced by ox-LDL,whieh may be the action mechanism of AT in anti-atherosclerosis and in inhibiting inflammatory factor.