诱导胶质纤维酸性蛋白阳性神经前体细胞系向神经元分化

目的：探讨来源于人胚胎脑室下区(SVZ)的胶质纤维酸性蛋白(GFAP)阳性神经前体细胞系向神经元分化的潜能。方法：分别采用定量RT-PCR、Western印迹分析及免疫荧光染色检测被全反式视黄酸诱导前后GFAP阳性神经前体细胞系中神经干细胞和神经元特异性标志物表达量的变化情况。采用免疫荧光染色检测GFAP阳性神经前体细胞系移植到动物体内后神经元特异性标志物表达情况。结果：在体外诱导后,GFAP阳性神经前体细胞系中神经元特异性标志物在mRNA和蛋白水平的表达量上升,而神经干细胞标志物表达量下降。移植动物体内,GFAP阳性神经前体细胞系可分化产生神经元。结论：GFAP阳性神经前体细胞系在体内外可以向神经元诱导分化。

The induction of neuronal differentiation in the glial fibrillary acid protein positive human neural progenitor cell line

Objective: To investigate the ability of human GFAP positive neural progenitor cell line from the subventricular zone(SVZ) to differentiate into neurons. Methods: Real time RT PCR, Western blot analysis and immunocytochemistry were used to examine the expression level of the neural stem cell marker and neuronal specific marker before and after all trans retinoic acid (AT RA) induction in the GFAP positive neural progenitor cell line. Immunocytochemistry was used to examine the expression of the neuronal specific marker after transplantation the GFAP positive neural progenitor cell line into the animal model. Results: After induction, in the GFAP positive neural progenitor cell line the expression levels of the neuronal specific marker increased, while the neural stem cell marker decreased both in mRNA and protein levels. After transplantation into animal model, the GFAP positive neural progenitor cell line could differentiate into neurons. Conclusion: The GFAP positive neural progenitor cell line could be induced to differentiate into neurons both in vitro and in vivo.