Dot hybridization and polymerase amplification of DNA in the prenatal diagnosis of cytomegalovirus infection

Molecular biology techniques (dot hybridization and polymerase amplification of DNA) are used to diagnose cytomegalovirus infection in pregnant women and newborns. It is concluded from the results that dot hybridization is a convenient tool in screening studies and that polymerase DNA amplification followed by verification of the reaction product using restriction enzymes and blot hybridization makes the diagnosis of this disease more reliable and may find wide application in practical laboratories. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N ^o 7, pp. 77–79, July, 1994 Presented by A. A. Totolyan, Member of the Russian Academy of Medical Sciences