抗CD94单抗与NK细胞、γδ^＋T细胞及CTL功能的关联

目的：探讨抗CD94单抗与NK细胞、γδ^ T细胞及CTL的相关性。方法，采用荧光检测法，分别分析NK细胞、γδ^ 细胞和CTL上CD94分子的表达，采用^3H TdR掺入法和MTT释放法，分析抗94单抗对这3种细胞的杀伤活性和细胞增殖的影响。结果NK细胞和γδ^ T细胞上CD94分子的表达，明显高于PBL和CTL。抗CD94单抗能激活NK细胞、γδ^ T细胞和CTL的增殖，并能提高γδ^ T细胞和CTL的杀伤活性。结论CD94分子在NK细胞和γδ^ T细胞表面呈高表达。抗CD94单抗对NK细胞、γδ^ T细胞及CTL的增殖具有上调节作用，对它们的杀伤活性具有正向调节作用。

Modulation of theproliferation and cytotoxicities of NK,

Aim To explore the correlation of anti-CD94 mAb with the NK cell,γ δ ＋ T cell and CTL proliferation and cytotoxicities. Methods The lymphocytic proliferations, CD94 molecular expression and the effect of anti-CD94 mAb on their cytotoxic activities were studied by 3H-TdR incorporation assay, immunoflurescence assay and MTT test,respectively. Results CD94 molecular expressions on NK and γ δ ＋ T cells were obviously higher than those on PBL.Anti CD94 mAb could accelerate three lymphocytic proliferation and enhance their cytotoxicities. Conclusions CD94 molecules are highly expressed on the NK and γ δ ＋ T cells. The molecule plays the roles of accelerating the proliferation the three types of lymphocyte and enhancing the cytotoxicities of the γ δ ＋ T cells and CTL