三七总皂苷对AngⅡ诱导心肌细胞凋亡的影响

目的:研究三七总皂苷对血管紧张素Ⅱ(AngⅡ)诱导培养心肌细胞凋亡的影响。方法:原代培养乳鼠心肌细胞,通过MTT法观察三七总皂苷对培养心肌细胞活力的影响;AO荧光染色观察细胞核形态改变,流式细胞术检测细胞凋亡,Fluo3荧光探针标记测定钙离子荧光强度。结果:AngⅡ(10^-7mol·L^-1)孵育48h后心肌细胞凋亡明显多于对照组;三七总皂苷(25,50,100mg·L^-1)组心肌细胞活力显著高于AngⅡ组;三七总皂苷(50mg·L^-1)组AngⅡ诱导的心肌细胞凋亡率明显低于AngⅡ组;三七总皂苷(50mg·L^-1)组心肌细胞内钙超载明显轻于AngⅡ组。结论:三七总皂苷对AngⅡ诱导的细胞凋亡具有明显的抑制作用,其机制可能与抑制心肌细胞内钙超载有关。

Effects of Panax notoginseng saponins on rat cardiomyocytes apoptosis induced by angiotengin Ⅱ in vitro

OBJECTIVE: To study the protective effects of Panax notoginseng saponins (PNS) on angiotensin II (Ang II)-induced rat cardiomyocyte apoptosis in vitro and the probable mechanism. METHOD: Cultured cardiomyocytes from neonatal rats were stimulated with Ang II. Cell viability was measured by MTT. Apoptosis was evaluated using Acridine Orange (AO) fluorescent dye staining and flow cytometry; Fluo-3 AM was used to test the change of intracellular free calcium. RESULT: It was found that incubating with Ang II (10(-7) mol x L(-1)) for 48 h increased cardiomyocyte apoptosis, PNS (25, 100 mg x mL(-1)) increased myocyte viability. PNS (50 mg x mL(-1)) significantly decreased this Ang II-induced rat cardiomyocyte apoptosis (P < 0.05) and decreased fluorescent intensity of intracellular calcium. CONCLUSION: PNS has a significant effect on Ang II-induced rat cardiomyocytes apoptosis in vitro by alleviating intracellular calcium overload.