| RAPD技术鉴定商品药材百合 |
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| 引用本文： | 林丽美,刘塔斯,肖冰梅,王燕,王晟.RAPD技术鉴定商品药材百合[J].湖南中医药大学学报,2006,26(5):30-32. |
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| 作者姓名： | 林丽美 刘塔斯 肖冰梅 王燕 王晟 |
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| 作者单位： | 1. 湖南中医药大学,湖南,长沙,410007
2. 中国中医研究科学院,北京,100700 |
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| 基金项目： | 湖南省卫生厅中医药科研项目 |
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| 摘 要： | 目的鉴别商品药材百合及其混淆品,探讨商品药材鉴别的新方法。方法采用RAPD技术对商品药材百合进行了多态性分析,并构建聚类树型图。结果RAPD技术能从分子水平上检测种内差异,可以解决不同产地的商品药材百合与混淆品轮叶百合不易区分的难题。结论RAPD技术完全可以作为商品药材的一种较好的鉴别方法。
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| 关 键 词： | RAPD技术 百合 遗传多态性 药材鉴定 |
| 文章编号： | 1000-5633（2006）05-0030-03 |
| 收稿时间： | 2006-04-17 |
| 修稿时间： | 2006-04-17 |
Identification of Commercial Crude Drug Bulbus Lilii with RAPD Technique |
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| LIN Li-mei,LIU Ta-si,Xiao bing-mei,Wang yan,Wang sheng.Identification of Commercial Crude Drug Bulbus Lilii with RAPD Technique[J].Journal of Traditional Chinese Medicine University of Hunan,2006,26(5):30-32. |
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| Authors: | LIN Li-mei LIU Ta-si Xiao bing-mei Wang yan Wang sheng |
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| Institution: | 1. China Academy of Traditional Chinese Medicine, Beijing 100700, China; 2. TCM University of Hunan, Changsha 410007, China |
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| Abstract: | Objective To identify the commercial medicinal materials Bulbus Lilii and the confused materials,and investigate a new identifying method for the medicinal materials.Methods Polymorphic analysis was used for the commercial crude drug Bulbus Lilii with RAPD technique and a tree diagram was constructed.Result The RAPD technique could detect the differences between the different materials within the molecule elevel and solve the difficult problem of the commercial crude drug Bulbus Lilii confused with the other ones.Conclusion The RAPD technique can be taken as a effective identification for commercial crude drug Bulbus Lilii. |
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| Keywords: | RAPD technique Bulbus Lilii genetic polymorphics identification of medicinal materials |
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