Survivin蛋白表达与鼻咽癌临床特征相关性研究

目的:通过采用免疫组化的方法,检测鼻咽癌组织中以及鼻咽黏膜慢性炎症组织中Survivin蛋白的表达,探讨该蛋白在鼻咽癌发生发展中的作用和意义。方法:应用免疫组化链亲和素-过氧化酶法(SP法)检测105例患者鼻咽癌组织中放疗前Survivin的表达情况,取10例正常鼻咽黏膜标本做对照。所有患者均行首程根治性放疗。随访5至7年,随访率95%。所有资料经SPSS13.0软件统计包处理,进行χ2检验、Cox回归、生存分析。结果:①105例鼻咽癌组织中Survivin阳性表达率分别为58%,与正常鼻咽组织中阳性表达率相比较,差异均有统计学意义(P<0.05);②Survivin蛋白的表达与性别、年龄、淋巴结转移、肿瘤分期、颅神经麻痹、颅底侵犯、茎前与茎后侵犯、VCA-IgA均无明显相关性(P>0.05)。③临床上,Survivin蛋白阴性表达比阳性表达有较高的5年生存率,低表达比高表达有较高的5年生存率,但生存曲线比较无统计学意义(P>0.05)。④Cox回归模型表明Survivin蛋白的表达对鼻咽癌患者生存时间无明显影响(P>0.05)。结论:Survivin蛋白在对鼻咽癌中有较高的表达,其与肿瘤的临床特征关系不是十分密切,虽然Survivin蛋白表达均阴性的鼻咽癌患者较阳性表达的患者有更长的5年生存率,但要得到可信的结论仍需要较多病例数,较长时间的观察。

Study on correlation between nasopharyngeal carcinoma and expression of Survivin protein

ObjectiveTo investigate the expression of Survivin protein in nasopharyngeal carcinoma and nasopharyngeal mucosa tissue of chronic inflammation by the way of immunohistochemistry staining,in order to approach the protein effect and significance of the occurrence and development in nasopharyngeal carcinoma and hope to give theoretical guidance to the diagnosis,therapy and prognosis in the clinic practice of nasopharyngeal carcinoma.MethodImmunohistochemistry was used to determinate the expression of Survivin protein in tissue in 105 nasopharyngeal carcinoma patients,which contrasted with 10 cases of normal nasopharyngeal tissue.All the patients had received radical radiotherapy first.Followed up for 5 ~ 7 years,the rate of following up was by 95%.The methods that were used to analyze the data were chi-squre test,Cox regression,survival analysis.The analysis was done by SPSS version 13.0 software.Results①The positive expression of Survivin protein in nasopharyngeal carcinoma are 58%,which has statistical significance between carcinoma group and normal tissue group.②Intensity of the expression of Survivin protein has no significant correlation with gender,age,lymph nodes metabasis,tumor stage,cranial nerve palsy,basalis encroachment,encroachment in front or back of the belemnoid,VCA-IgA （P〉0.05）.③The negative expression of Survivin protein has better 5-year survival rate than positive expression of it,low expression of it has better 5-year survival rate than high expression in clinic,however the survival function has no evident significance in statistics among the groups （P〉0.05）.④Cox regression indicates that the expression of Survivin protein is not evidently associated with the survival of the patients with nasopharyngeal carcinoma （P〉0.05）.Conclusion①The abnormal expression of Survivin plays a certain role in course of the occurrence and development of nasopharyngeal carcinoma.②The study with more cases and longer time observing are necessary for the effect of prognosis coming from Survivin gene in nasopharyngeal carcinoma patients,though the negative expression of Survivin protein has better survival rate than the positive expression in clinic.