新型器官保存液低温保存猪肾效果的研究

目的研究新型器官保存液Lifor液对猪肾脏的低温保存效果。方法 24只白色杂种猪,随机均分为Lifor液保存组和威斯康星大学保存液（UW液）组各12只,建立离体肾脏非循环灌注模型,供肾取出后分别以0~4℃的Lifor液和UW液灌注并低温保存,再根据保存时间随机分成2个亚组,分别为保存24h、48h组,然后行猪自体肾移植。比较两组肾脏低温保存结束后其病理学及肾皮质三磷腺苷（ATP）含量的改变,并观察自体肾移植后肾功能恢复情况。结果供肾离体保存24h、48h后,Lifor液组与UW液组的肾组织病理学改变基本一致,肾皮质ATP含量比较差异无统计学意义（P〉0.05）。移植后两组血清肌酐水平比较差异亦无统计学意义（P〉0.05）。结论 Lifor液低温保存肾脏的效果与UW液相当,且成本低廉,因此更具有临床应用前景。

Cryopreservation of canine kidneys in new-type organ preservation solution:compared with UW solution

Objective To study the cryopreservation effect of Lifor organ preservation solution in preserving pig kidneys.Methods A total of 24 pigs were randomly divided into two groups：Lifor group and UW group.The isolated non-circulated perfused kidney models were created.Kidneys in Lifor group and UW group were respectively preserved in Lifor solution and UW solution for 24 and 48 hours in 0~4 ℃ before pig kidney auto-transplantation.The pathology and adenosine triphosphate（ATP）change of renal cortex were compared between Lifor and UW group.And the kidney function changes were observed after auto-transplantation.Results After 24 h and 48 h cold preservation,there was no significant differences in pathological changes,the content of ATP of kidney cortex as well as kidney function between the two groups（all in P0.05）.Conclusion The cryopreservation effect of Lifor solution is similar to UW solution but less cost.It may be developed as a new cryopreservation solution for clinical application.