HPLC测定鱼腥草不同部位绿原酸和芦丁的含量

目的:测定鱼腥草不同部位中绿原酸和芦丁的含量,为鱼腥草资源的合理开发和综合利用提供依据.方法:采用Eclipse XDB-C18(4.6mm×150mm,5μm)色谱柱,绿原酸流动相乙腈-0.1％磷酸(12∶88),检测波长326 nm,芦丁流动相甲醇-0.1％磷酸(50∶50),检测波长355 nm,流速均为1.0 mL· min-1.结果:绿原酸和芦丁的浓度与峰面积,分别在9.8 ～68.6μg(r =0.999 7),9.6～67.2μg(r=0.998 5)呈良好的线性关系;平均加样回收率分别为99.2％,100.1％,RSD分别为1.47％,2.12％.结论:鱼腥草不同部位绿原酸和芦丁含量差异很大,叶中绿原酸和芦丁含量相对较高,根中绿原酸和芦丁含量相对较低.

Determination of Chlorogenic Acid and Rutin in Different Parts of Houttuynia Cordatas by HPLC

Objective:To determine the content in roots,stems and leaves of Houttuynia cordata,and provide a scientific basis for the exploitation and utilization of its resources.Method:Chlorogenic acid and rutin were analyzed on a Eclipse XDB-C18(4.6 mm×150 mm,5 μm)column.The mobile phase of chlorogenic acid was acetonitrile-0.1% phosphoric acid(12∶88),and the detection wavelength was set at 326 nm.The mobile phase of rutin was methanol-0.1% phosphoric acid(50∶50),and the detection wavelength was set at 355 nm.The flow rate was 1.0 mL · min-1.Result:The linear response ranged within 9.8-68.6 μg for chlorogenic acid(r=0.999 7),9.6-67.2 μg for rutin(r=0.998 5).Recoveries were 99.2% with RSD 1.47% for chlorogenic acid,100.1% with RSD 2.12% for rutin.Conclusion:There was a significant difference in the content of chlorogenic acid and rutin among the different parts of H.cordata.The content of chlorogenic acid and rutin in the sample from leaves was the highest than those from other parts,and it was lower in roots relatively.