严重急性呼吸综合征患儿血清抗严重急性呼吸综合征相关冠状病毒免疫球蛋白G远期随访

目的远期随访严重急性呼吸综合征(SARS)患儿血清中特异性抗SARS相关冠状病毒(CoV)IgG(SARS-CoV-IgG)抗体,并探讨其临床意义。方法儿童SARS临床诊断病例16例和患儿的父母及与其密切接触家庭成员(其中包括7例SARS临床诊断病例、12例非SARS病例)19例。在SARS患儿病程1.5年左右,进行随访。采集患儿及其密切接触家庭成员外周静脉血2 mL,常规分离血清。应用间接免疫荧光法(IFA)检测受试者血清中抗SARS-CoV-IgG抗体。结果16例儿童SARS病例中,8例血清中抗SARS-CoV-IgG阳性,其中4例SARS患病期间抗SARS-CoV-IgM阳性,4例未查抗SARS-CoV-IgM抗体。另8例血清中抗SARS-CoV-IgG阴性,其中4例SARS患病期间抗SARS-CoV-IgM阴性,4例未查抗SARS-CoV-IgM抗体。8例血清中抗SARS-CoV-IgG阳性的SARS患儿的8个家庭中均有2例及2例以上SARS患者。8例血清中抗SARS-CoV-IgG阴性的儿童SARS病例的8个家庭中均只有患儿本人为SARS患者。结论在病程1.5年后,实验室确诊的儿童SARS病例的血清中仍能检测到抗SARS-CoV-IgG抗体。家庭聚集发病是实验室确诊的SARS病例的一个重要特征。

Long- Term Follow- Up of Specific Immunoglobulin G against Coronavirus Associated Severe Acute Respiratory Syndrome in Children with Servere Acute Respiratory Syndrome

Objective To investigate the long-term presence of specific immunoglobulin G(IgG) against coronavirus associated severe acute respiratory syndrome(SARS-CoV-IgG) and explore the significance of SARS-CoV-IgG.Methods Sixteen pediatric clinical SARS cases and 19 members(including 7 adult SARS cases and 12 healthy adults) of their families were enrolled into this study.Serum samples were obtained from the patients and their family members one and a half year after onset of the disease.Serum SARS-CoV-IgG was detected by indirect immunofluorescent assay.Results Of 16 pediatric SARS cases,8 cases were positive of SARS-CoV-IgG and another 8(cases) were negative.Of 8 cases with positive of SARS-CoV-IgG,4 cases were positive of SARS-CoV-IgM during hospitalization and the other 4 cases had not been detected.Of 8 cases with negative of SARS-CoV-IgG,4 cases were negative of SARSCoV-IgM(during) hospitalization and the other 4 cases had not been detected.Of 8 patients with positive SARS-CoV-IgG,there were two or more SARS patients in their families respectively.Of 8 patients with negative SARS-CoV-IgG,there were the only patients in their families respectively.Conclusions SARS-CoV-IgG can be detected in sera of pediatric SARS cases associated with coronavirus one and a half year after onset of disease.Outbreak in family is an important characteristic of SARS associated with corona virus.