| 阿司匹林对人子宫内膜腺癌Ishikawa细胞生长和凋亡的影响 |
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| 引用本文： | 赵冬妮,金英楠,李艳霞,马晓欣. 阿司匹林对人子宫内膜腺癌Ishikawa细胞生长和凋亡的影响[J]. 中国医科大学学报, 2010, 39(2): 132-134 |
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| 作者姓名： | 赵冬妮 金英楠 李艳霞 马晓欣 |
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| 作者单位： | 1. 中国医科大学,附属盛京医院妇产科,沈阳,110004
2. 北京市西城区疾病预防控制中心,生殖保健科,北京,100029 |
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| 基金项目： | 辽宁省教育厅高校科研基金资助项目(05L477) |
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| 摘 要： | 目的研究阿司匹林对体外培养的人子宫内膜腺癌Ishikawa细胞增殖和凋亡的影响。方法采用MTT法检测不同浓度及作用时间下阿司匹林对子宫内膜腺癌Ishikawa细胞增殖的影响;通过流式细胞仪检测不同浓度阿司匹林对细胞周期和凋亡率的影响;应用透射电镜观察阿司匹林作用后细胞的形态学变化。结果阿司匹林对人子宫内膜腺癌Ishikawa细胞的增殖具有明显的抑制作用,呈时间与剂量依赖性(P<0.05);阿司匹林使细胞阻滞在G1期,细胞凋亡率增加,与药物作用浓度相关(P<0.05);阿司匹林作用后细胞体积缩小,染色质浓缩致密,可见凋亡小体形成。结论阿司匹林能够显著抑制人子宫内膜腺癌Ishikawa细胞的增殖,并诱导其发生凋亡。
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| 关 键 词： | 阿司匹林 Ishikawa细胞 肿瘤生长 子宫内膜腺癌 |
Effects of Aspirin on the Proliferation and Apoptosis of Human Endometrial Adenocarcinoma Ishikawa Cells |
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| ZHAO Dong-ni , JIN Ying-nan , LI Yan-xia , MA Xiao-xin. Effects of Aspirin on the Proliferation and Apoptosis of Human Endometrial Adenocarcinoma Ishikawa Cells[J]. Journal of China Medical University, 2010, 39(2): 132-134 |
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| Authors: | ZHAO Dong-ni JIN Ying-nan LI Yan-xia MA Xiao-xin |
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| Affiliation: | ZHAO Dong-ni1,JIN Ying-nan2,LI Yan-xia1,MA Xiao-xin1(1.Department of Obstetrics , Gynecology,Shengjing Hospital,China Medical University,Shenyang 110004,China,2.Department of Reproductive Health Care,Xicheng District Center for Disease Control , Prevention,Beijing 100029,China) |
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| Abstract: | Objective To assess the effects of aspirin on the proliferation and apoptosis of human endometrial adenocarcinoma cells.Methods MTT assay was used to measure the effects of aspirin on the proliferation of Ishikawa cell.Flow cytometry(FCM)was employed to examine the distribution of cell cycles and the rates of apoptosis.Transmission electron microscopy was used to observe cell morphologic changes after aspirin administration.Results Aspirin inhibited the proliferation of cultured Ishikawa cells in a time-dep... |
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| Keywords: | aspirin Ishikawa cell tumor growth endometrial adenocarcinoma |
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