局灶性脑缺血大鼠白细胞介素10的变化

探讨脑缺血后不同时间血清和局部脑缺血组织白细胞介素10的变化.采用改良Zea Longa线栓法制作大鼠大脑中动脉闭塞模型,在缺血不同时间,应用双抗体夹心间接酶联免疫吸附法检测缺血组和假手术对照组大鼠受损脑组织及血清中白细胞介素10的浓度.结果发现,缺血组脑组织白细胞介素10含量在大脑中动脉闭塞3h处于较低水平,与假手术对照组比较无明显变化(P＞0.05);6 h达最低,与假手术对照组比较变化显著(P＜0.01),随后逐渐升高(P＜0.05);血清中白细胞介素10含量在3h最低,与假手术对照组比较变化显著(P＜0.05),随后逐渐升高(P＜0.05).结果提示,白细胞介素10对脑缺血损伤可能有保护作用.

Change of Interleukin-10 in Middle Cerebral Artery Occlusion of Rats

Aim To study the change of interleukin-10 (IL-10) in focal ischemia tissue of brain and serum in middle cerebral artery occlusion (MCAO) of rats. Methods The middle cerebral artery (MCA) was occluded for different time with modified Zea Longa' s MCAO model. The level of interleukin-10 in focal ischemia tissue of brain and blood serum was measured with double-antibody sandwich enzyme linked immunosorbent assay (ELISA), both in ischemia group and in control group. Results The content of interleukin-10 in focal ischemia tissue of brain was lower at 3 h after ischemia, compared with the control (P > 0.05), and it fell to the lowest degree at 6 h, compared with the control (P < 0.01), then it increased compared with the control (P < 0.05). The concentration of interleukin-10 in serum fell to the lowest degree at 3 h after ischemia compared with the control (P<0.05), and then it increased slowly compared with the control (P< 0.05). Conclusion Interleukin-10 perhaps afford protection to the cerebral ischemia injury.