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| 槲皮素对NB4细胞株p53及蛋白的影响 | |
| 引用本文： | 谢庆文,赵劲秋,方智雯. 槲皮素对NB4细胞株p53及蛋白的影响[J]. 上海交通大学学报(医学版), 2001, 21(1) |
| 作者姓名： | 谢庆文 赵劲秋 方智雯 |
| 作者单位： | 1. 上海市铁路中心医院血液科, 2. 上海第二医科大学仁济医院血液科 |
| 摘 要： | 目的研究治疗白血病的有效药物。方法将槲皮素作用于培养的NB4细胞株，应用RT-PCR及Westem bolt方法，研究不同浓度槲皮素(30、60、90μmol/L)诱导细胞24、48、72、96、120h后，急性早幼粒细胞白血病NB4细胞株中突变型p53基因及蛋白表达的情况。结果证实其在30～90μmol/L浓度时对突变型p53基因无影响，而对突变型p53蛋白的表达有抑制作用。结论本研究为槲皮素应用于急性早幼粒细胞白血病的研究提供了有效手段。 |
| 关 键 词： | 槲皮素 NB4 突变型p53基因 突变型p53蛋白 |
The Effect of Quercetin Targeted p53 Gene and Protein Expression in NB4 Cell Lines |
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| Xie Qing-wen,ZHAO Jing-qiu,FANG Zhi-wen. The Effect of Quercetin Targeted p53 Gene and Protein Expression in NB4 Cell Lines[J]. Journal of Shanghai Jiaotong University:Medical Science, 2001, 21(1) | |
| Authors: | Xie Qing-wen ZHAO Jing-qiu FANG Zhi-wen |
| Abstract: | Objective In order to study the value of drug treatment for leukemia, quercetinwas delivered to the cultured NB4 cell line. Methods Using RT- PCR and western blot, we studiedthe expression of mutant p53 gene and protein after inducing 24, 48, 72, 96 and 120h by the quercetinwith different concentrations (30, 60, 90μmol/L). Results We verified the results that quercetinwith concentration of 30 ～90μmol/L could successfully inhibit the expression of mutant p53 protein,but the mutant p53 gene did not have. Conclusion That data suggested that quercetin would be aneffective method for the therapy of leukemia. |
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