外源多胺对大鼠早期再生肝鸟氨酸脱羧酶蛋白水平的调节

目的 研究多胺(腐胺、精脒和精胺)对大鼠再生肝鸟氨酸脱羧酶(ODC)蛋白水平的调节,分析多胺在肝再生中的作用.方法 外源多胺(溶于0.9%NaCl)皮下注射雄性SD大鼠(180～220 g),部分肝切除(PH)诱导的大鼠再生肝中ODC蛋白水平的分析采用免疫印迹(Western blotting)方法.结果 高剂量的腐胺(20 ms/kg体重)、精脒(0.15 mg/kg体重)和精胺(6 mg/kg体重)处理后,ODC蛋白水平在PH后12 h内均低于对照组,且各组间变化趋势相近;而低剂量腐胺(0.02 mg/kg体重)、精脒(0.03 mg/kg体重)及精胺(0.06 ms/kg体重)处理组在PH后4h ODC水平迅速升高,分别比对照组高15.1%、29.5%和30.3%.结论 一定剂量的多胺对大鼠早期再生肝ODC蛋白水平有反馈调节作用,其中精脒、精胺的作用较强,腐胺较弱.

THE REGULATION OF EXOGENOUS POLYAMINES ON ODC PROTEIN LEVEL IN THE EARLY REGENERATING LIVER OF RATS

Objective To study the role of polyamines (putrescine, spermidine and spermine) in regulating ornithine decarboxylase (ODC) protein levels in the early regenerating liver of rats. Methods Exogenous polyamines (dissolved in 0.9% NaCl) were administered subcutaneously to adult male SD rats (180～220g). The ODC protein levels in the regenerating liver induced by partial hepatectomy (PH) were measured by Western blotting analysis. Results The treatment with high doses of putrescine ( 20 mg/kg body weight), spermidine (0.15 mg/kg body weight) or spermine ( 6 mg/kg body weight) reduced the ODC protein level compared with the control group within 12 hours after PH. However,the treatment with low doses of putrescine (0.02 mg/kg), spermidine (0.03 mg/kg) or spermine (0.06 mg/kg) resulted in a rapid increase in the protein level in the 4th hour after PH, which was 15.1%, 29.5% or 30.3% higher than that of control rats respectively.Conclusion Polyamines with certain doses play a role in the feedback regulation of ODC protein levels in the early regenerating liver, where spermidine and spermine play a greater role than putrescine dose.