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| 角质形成细胞μ-阿片受体表达的研究 | |
| 引用本文： | 毕新岭,顾军,米庆胜,田中伟. 角质形成细胞μ-阿片受体表达的研究[J]. 临床皮肤科杂志, 2005, 34(3): 137-138 |
| 作者姓名： | 毕新岭 顾军 米庆胜 田中伟 |
| 作者单位： | 1. 第二军医大学长海医院皮肤科,上海,200433 2. 泰山医学院附属医院皮肤科,山东,泰安,271000 3. 西安交通大学第二医院皮肤科,陕西,西安,710004 |
| 基金项目： | 国家自然科学基金资助项目(30271197) |
| 摘 要： | 目的：研究不同特性的角质形成细胞μ-阿片受体的表达情况。方法：以体外培养的角质形成细胞株HaCaT细胞、人鳞状细胞癌(简称鳞癌)细胞株A431、神经母细胞瘤SK-N-SH细胞株为对象，采用逆转录(RT)-PCR方法研究细胞μ-阿片受体的表达。结果：在常规体外培养条件下，角质形成细胞株HaCaT细胞、人鳞癌细胞株A431的RT-PCR结果显示有μ-阿片受体mRNA的表达，后者的表达水平略高于前者。结论：μ-阿片受体在角质形成细胞的表达，为神经系统和皮肤通过神经肽直接发生作用提供依据。 |
| 关 键 词： | 角质形成细胞 μ-阿片受体 |
| 文章编号： | 1000-4963(2005)03-0137-02 |
| 修稿时间： | 2004-05-24 |
The expression of mu-opiate receptor in human keratinocyte cell lines |
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| BI Xin-ling,GU Jun,MI Qing-sheng,TIAN Zhong-wei. The expression of mu-opiate receptor in human keratinocyte cell lines[J]. Journal of Clinical Dermatology, 2005, 34(3): 137-138 | |
| Authors: | BI Xin-ling GU Jun MI Qing-sheng TIAN Zhong-wei |
| Abstract: | Objective: To study the expression of mu-opiate receptor in different human cell lines. Methods: The cells of human epidermal keratinocyte cell line (HaCaT), human squamous carcinoma cell line (A431) and neuroblastoma cell line (SK-N-SH) were cultured in a conventional culture-condition. The expression of mu-opiate receptor in cell lines was assessed by RT-PCR method. Results: The expression of mu-opiate receptor mRNA was found in the HaCaT and A431, and the expression level was higher in A431 than that in HaCaT. Conclusion: The expression of mu-opiate receptor mRNA in the keratinocytes indicates that the nervous system may interact with the skin by the neuropeptides.[Key words] keratinocytes; mu-opiate receptor |
| Keywords: | keratinocytes mu-opiate receptor |
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