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| ELISA一步法与TPPA法检测梅毒螺旋体抗体应用分析 | |
| 引用本文： | 王海滨,林霞,段慧玲,李忠俊. ELISA一步法与TPPA法检测梅毒螺旋体抗体应用分析[J]. 重庆医学, 2009, 38(12) |
| 作者姓名： | 王海滨 林霞 段慧玲 李忠俊 |
| 作者单位： | 解放军总医院第一附属医院检验科,北京,100048;第三军医大学新桥医院输血科,重庆,400037 |
| 摘 要： | 目的 探讨酶联免疫吸附实验(ELISA)一步法与梅毒螺旋体明胶颗粒凝集试验(TPPA)检测梅毒螺旋体抗体的优劣.方法 对2007年1月至2009年3月的51 065份血清标本分别进行TPPA法和ELISA一步法检测,并对检测阳性不一致的标本采用稀释法进行ELISA检测.结果 TPPA法检测梅毒螺旋体抗体阳性1 152例(2.26%),ELISA一步法检测阳性1 117例(2.19%),差异无统计学意义(P>0.05),但ELISA一步法检测出现灰区标本35例经TPPA法复查和倍比稀释后用ELISA法检测结果均为阳性,确证阳性率为100%.结论 TPPA法检测梅毒螺旋体抗体能避免ELISA一步法引起的前带现象,并且对ELISA一步法检测梅毒螺旋体抗体时出现的灰区标本进行TPPA法复查,可提高梅毒螺旋体抗体检出率. |
| 关 键 词： | 酶联免疫吸附实验 明胶颗粒凝集试验 梅毒 |
Clinically analysis of detecting syphilis antibodies by sandwich ELISA and TPPA |
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| WANG Hai-bin,LIN Xia,DUAN Hui-ling,et al.. Clinically analysis of detecting syphilis antibodies by sandwich ELISA and TPPA[J]. Chongqing Medical Journal, 2009, 38(12) | |
| Authors: | WANG Hai-bin LIN Xia DUAN Hui-ling et al. |
| Affiliation: | WANG Hai-bin1,LIN Xia2,DUAN Hui-ling2,et al.(1.Department of Lab Medicine,the General Hospital PLA,Beijing 100048,China,2.Department of Blood Transfusion,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China) |
| Abstract: | Objective To investigated the advantages and defects of sandwich ELISA and TPPA,used for detecing syphilis antibodies.Methods The 51 065 serum specimens were collected from Jan 2007 to Mar 2009.ELISA and TPPA were applied in syphilis antibodies detection.Results In 51 065 specimens,1 152(2.26%)of them were positive with the method of TPPA and 1 117(2.19%) of them were positive with the method of sandwich ELISA.All specimens,the S/CO value of which between 0.5 to 1.0 in sandwich ELISA,were confirmed to be po... |
| Keywords: | enzyme-linked immunosorbent assay powdered gelatine agglutination test syphilis |
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