眼睑基底细胞癌中整合素αvβ3、Tmem16a 表达及其与血管内皮生长因子的相关性

探讨整合素αvβ3与跨膜蛋白16a（Tmem16a）在眼睑基底细胞癌中的表达及其与血管内皮生长因子（VEGF）的相关性。方法：实验研究。选择2013年5月至2017年5月郑州人民医院眼科72份确诊的眼睑基底细胞癌术后组织为研究组，另取经病理证实的癌旁正常组织40份作为对照组，运用免疫组化法测定其整合素αvβ3、Tmem16a、VEGF蛋白表达情况，分析整合素αvβ3、Tmem16a、VEGF 蛋白表达情况与患者临床特征的关系，探讨整合素αvβ3、Tmem16a蛋白与VEGF的相关性。采用t检验或χ2 检验进行组间比较，采用单因素Logistic回归分析αvβ3、Tmem16a及VEGF表达的影响因素，采用Pearson线性相关分析其相关性。结果：研究组整合素αvβ3、Tmem16a、VEGF阳性表达率均明显高于对照组（χ2 =16.94、24.38、23.94，P<0.05）。研究组整合素αvβ3、Tmem16a、VEGF阳性率的影响因素均包括增殖细胞核抗原增殖指数、肿瘤最大直径（P<0.05），而与患者性别、年龄无相关性（P>0.05）。眼睑基底细胞癌患者的癌组织中整合素αvβ3、Tmem16a的表达与VEGF表达呈正相关（r=0.642、0.516，P<0.001）。结论：整合素αvβ3、Tmem16a在眼睑基底细胞癌中高表达，且与肿瘤最大直径、增殖细胞核抗原增殖指数相关，其可能通过促进VEGF表达促进血管新生，加快眼睑基底细胞癌的发生及进展。

Expression of Integrin αvβ3 and Tmem16a and Their Correlation with Vascular Endothelial Growth Factor in Eyelid Basal Cell Carcinoma

To investigate the expression of Integrin alpha V/beta 3 (αvβ3) and transmembrane protein 16A (Tmem16a) in basal cell carcinoma of the eyelid and its correlation with vascular endothelial growth factor (VEGF). Methods: In this experimental study, 72 cases of basal cell carcinoma of eyelid after operation were enrolled in Zhengzhou People's Hospital from May 2013 to May 2017 as the study group, another 40 cases of normal tissue around the cancer as the control group, using immunohistochemical method to determine the expression of Integrin αvβ3, Tmem16a, VEGF protein, and to explore the relationship between Integrin αvβ3, Tmem16a protein and VEGF. The data were analyzed by t test, chi-square test, Logistic regression and Pearson correlation. Results: The positive rates of Integrin αvβ3, Tmem16a and VEGF in the study group were significantly higher than those in the control group (χ2 =16.94, 24.38, 23.94, P<0.05). The positive rates of Integrin αvβ3, Tmem16a and VEGF were related to proliferating cell nuclear antigen (PCNA) and tumor diameter (P<0.05), but not to the sex and age of patients (P>0.05). The expression of Integrin αvβ3 and Tmem16a was positively correlated with the expression of VEGF in eyelid basal cell carcinoma (r=0.642, 0.516, P<0.001). Conclusions: Integrin αvβ3 and Tmem16a in basal cell carcinoma in the presence of high expression, and correlated with tumor diameter and PCNA proliferation index, which could promote the expression of VEGF, accelerate the occurrence and progression of basal cell carcinoma of eyelid.