A comparison of the haemagglutinating and enzymic activities ofBacteroides fragiliswhole cells and outer membrane vesicles |
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| Affiliation: | 1. Super Insulation Composite Laboratory, College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China;2. Center of Materials Physics and Chemistry, School of Physics and Nuclear Energy Engineering, Beijing University of Aeronautics and Astronautics, Beijing 100191, China;3. College of Materials Science and Engineer, Nanjing Tech University, Nanjing 211800, China;1. Institute of Medical Physics, Biophysics, Informatics and Telemedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia;2. Faculty of Natural Sciences, University of SS. Cyril and Methodius, Trnava, Slovakia;3. International Laser Centre, Bratislava, Slovakia;4. Department of Physical and Theoretical Chemistry, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia |
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| Abstract: | ![]()
The haemagglutinating and enzymic activities of the obligately anaerobic pathogenic bacteriumBacteroides fragiliswere examined. Outer membrane vesicles are released from the surface ofB. fragilis. They can be detected by electron microscopy in ultrathin sections and bacterial suspensions after negative staining. Electron microscopy and immunogold labelling with a MAb specific for surface polysaccharide ofB. fragilisconfirmed that the vesicles carried outer membrane associated epitopes. The haemagglutinating activity of whole cells from populations ofB. fragilisstrains NCTC9343, BE3 and LS66 enriched by Percoll density gradient centrifugation for a large capsule (LC), electron dense layer (EDL; non-capsulate by light microscopy) and outer membrane vesicles (OMV) which had been purified by centrifugation from EDL-enriched populations were compared using human and horse erythrocytes. The enzymic activity of OMV, LC- and EDL-enriched populations, as detected by the API ZYM kit, was compared for strains NCTC 9343 and BE3. Purified OMV from the strains examined exhibited both haemagglutinating and enzymatic activity. Haemagglutination by the EDL-enriched population was sensitive to treatment with sodium periodate. The LC-enriched population haemagglutinated only after ultrasonic removal of the capsule. This indicates that the LC masks a haemagglutinin. The results suggest a potential role for OMV in the virulence ofB. fragilis. |
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