GSTT1及GSTM1和CYP2E1基因多态性与二甲基甲酰胺所致肝脏损害的关系

目的 探讨GSTT1、GSTM1和CYP2E1基因多态性与二甲基甲酰胺(DMF)作业人员肝脏损害的关系.方法 以某皮革厂69名肝功能异常的DMF作业人员为病例组,选取与病例组相似岗位、DMF接触水平相近而肝功能正常的125名工人为对照组,应用聚合酶链反应(PCR)、PCR-RFLP方法分别对GSIT1、GSTM1和CYP2E1 PstI位点基因进行分型.结果 病例组和对照组中GSTM1阳性和GSTM1阴性的分布频率分别为59.42%、38.40%和40.58%、61.60%,两组间分布的差异有统计学意义(P=0.005),携带GSTM1阳性基因型个体发生肝功能异常的风险是GSTM1阴性基因型个体的2.34倍(OR=2.34,95%CI:1.29～4.29).而GSTT1和CYP2E1 PstI位点不同基因型在病例组和对照组中没有明显差异.结论 携带GSTM1阳性基因型个体的DMF作业人员发生肝功能异常的风险性可能增加.

Abnormal liver function associated with polymorphism of GSTT1,GSTM1 and CYP2E1 in workers exposed to N,N- dimethylformamide

Objective To investigate abnormal liver function associated with polymorphism of GSTT1,GSTM1 and CYP2E1 in workers exposed to N,N- dimethylformamide.Methods Sixty-nine workers with abnormal liver function in a synthetic leather factory were recruited as case.One hundred and twenty five control subjects with similar work tasks were selected from the same factory.Genotypes for GSTT1 and GSTM1 were determined by multiplex PCR,and for CYP2E1 PstI by PCR-RFLP assay.Results The frequency of positive GSTM1 was 59.42% in cases and 38.40% in control,with an odds ratio (OR) of 2.34,95% CI:1.29～4.29 (P=0.005).For GSTT1 and CYP2E1 PstI,the frequencies of genotypes showed no significant difference between case and control.Conclusion GSTM1 positive genotype may be genetic risk factors for development of abnormal liver function in workers exposed to N,N- diroethylformamide.