缺氧对成熟脂肪细胞去分化影响的实验研究

目的 研究缺氧环境下成熟脂肪细胞体外培养的形态学变化,探讨缺氧环境对成熟脂肪细胞去分化的影响.方法 从成人吸脂术后抽吸物提取脂肪组织来源的干细胞,诱导为成熟脂肪细胞,在缺血缺氧的环境下培养.观察各时间段细胞的形态变化.结果 由脂肪组织来源的干细胞诱导而成的成熟脂肪细胞,在缺氧环境培养数日后,细胞从圆形变为可持续分裂的成纤维细胞状细胞.结论 成熟的脂肪细胞在一定条件下可以去分化为成纤维细胞状细胞.

Effect of hypoxia on dedifferentiation of mature adipocytes: an experimental study

OBJECTIVE: To study the morphological changes of mature adipocytes in hypoxic condition in vitro and investigate the effect of hypoxia on dedifferentiation of mature adipocytes. METHODS: Human adipose tissue was collected from individuals undergoing liposuction procedures, from which the stem cells were isolated and induced to become mature adipocytes. After cell culture in hypoxic condition, the adipocytes were observed for morphological changes at different time points. RESULTS: After a few days of culture in hypoxic condition, the mature adipocytes derived from the adipose tissue-derived stem cells underwent morphological changes from spherical cells into fibroblast-like cells capable of continuous cell division. CONCLUSION: Mature adipocytes may dedifferentiate into fibroblast-like cells under appropriate conditions.