| 吴茱萸碱联合RO3306对人肝癌细胞HepG2的协同抑制 |
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| 引用本文： | 何培泳,谭宇蕙,吴映雅,张广献,杜标炎,王剑,李药菊.吴茱萸碱联合RO3306对人肝癌细胞HepG2的协同抑制[J].广州中医药大学学报,2011(5):503-507,565,566. |
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| 作者姓名： | 何培泳 谭宇蕙 吴映雅 张广献 杜标炎 王剑 李药菊 |
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| 作者单位： | 广州中医药大学基础医学院,广东广州,510006 |
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| 基金项目： | 国家自然科学基金资助项目(编号:30973811) |
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| 摘 要： | 目的]探讨吴茱萸碱(EVO)联合细胞周期蛋白依赖性激酶1(CDKl)的特异抑制剂RO3306对人肝癌细胞HepG2的生长抑制、诱导凋亡是否具有协同增效作用.方法]采用四甲基偶氮唑盐(MTT)法、碘化丙啶(PI)单染流式细胞术检测EVO诱导不可逆凋亡的时间转折点;采用MTT法、集落形成法、PI单染流式细胞术检测EVO...
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| 关 键 词： | 吴茱萸碱/药理学 肝癌/中西医结合疗法 细胞凋亡 细胞培养 |
Synergistic Inhibition of Evodiamine Combined with RO3306 on Human Hepatocarcinoma Cell Line HepG2 |
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| HE Peiyong,TAN Yuhui,WU Yingya,ZHANG Guangxian,DU Biaoyan,WANG Jian,LI Yaoju.Synergistic Inhibition of Evodiamine Combined with RO3306 on Human Hepatocarcinoma Cell Line HepG2[J].Journal of Guangzhou University of Traditional Chinese Medicine,2011(5):503-507,565,566. |
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| Authors: | HE Peiyong TAN Yuhui WU Yingya ZHANG Guangxian DU Biaoyan WANG Jian LI Yaoju |
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| Institution: | HE Peiyong,TAN Yuhui,WU Yingya,ZHANG Guangxian,DU Biaoyan,WANG Jian,LI Yaoju(School of Basic Medical Science,Guangzhou University of Chinese Medicine,Guangzhou 510006 Guangdong,China) |
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| Abstract: | Objective To investigate the proliferation inhibition and the apoptosis-induction effect of evodiamine(EVO) combined with RO3306,a specific CDK1 inhibitor,on human hepatocarcinoma cell line HepG2,and to study whether the effects are synergistic.Methods Methyl thiazolyl tetrazolium(MTT) assay,and propidium iodide(PI) staining associated with flow cytometry(FCM) assay were used to observe the occurrence of an irreversible apoptosis induced by EVO.MTT assay,colony-forming assay and FCM assay were used to inves... |
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| Keywords: | EVODIAMINE/pharmacology LIVER CANCER/TCM-WM therapy APOPTOSIS CELL CULTURE |
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