单侧隐睾大鼠对侧睾丸的损害与Bcl-2和Bax基因表达

目的:探讨单侧隐睾大鼠对侧睾丸生精细胞凋亡与Bcl-2/Bax基因表达的关系。方法:20只健康SD雄性大鼠(22日龄)随机分成隐睾组和对照组,每组10只。通过手术建立单侧隐睾动物模型。术后90 d取对侧睾丸,采用原位缺口末端标记(TUNEL)法检测生精细胞凋亡,免疫组化SP法检测Bcl-2/Bax基因表达。结果:与对照组相比,隐睾组对侧睾丸生精细胞凋亡显著增多(P<0.01),重量显著减轻(P<0.01),Bax表达显著升高(P<0.01),Bcl-2表达显著降低(P<0.01)。凋亡细胞主要是初级精母细胞和圆形精子细胞。结论:单侧隐睾大鼠对侧睾丸的生精细胞凋亡增多与Bcl-2基因表达降低、Bax基因表达升高密切相关。细胞内Bc l-2/Bax比值是影响生精细胞凋亡的重要因素之一。

Expression of Bcl-2 and Bax in the Impairment of Contralateral Testes of Unilateral Cryptorchidism in Rats

Objective: To investigate the relationship between germ cell apoptosis and expressions of Bcl-2 and Bax in contralateral testes of experimental unilateral cryptorchid rats.Methods: Twenty male Sprague-Dawley rats were randomly divided into 2 groups(namely cryptorchid group and control group),with 10 rats in each group.Cryptorchid animal model was established,and contralateral testes were corptured 90 days later.The evidence of germ cell apoptosis was detected by TUNEL assay.The expressions of Bcl-2 and Bax were detected by immunohistochemical method. Results: In the contralateral testes of experimental unilateral cryptorchidism,apoptosis index of germ cell and Bax expression significantly increased compared with those in the control group,respectively(P<(0.01)),while Bcl-2 expression and testis weight obviously decreased(P<(0.01)).The apoptotic cells were mostly pechytere spermatocytes and round spermatides.Conlusion: The germ cell apoptosis is highly correlated with expressions of Bcl-2 and Bax in contralateral testes of experimental unilateral cryptorchidism.Bcl-2/Bax plays an important role in germ cell apoptosis.