骨髓基质细胞分泌物促进体外脊髓运动神经元的存活和生长

目的体外检测骨髓基质细胞(Bonemarrow stromal

Bone marrow stromal cells secretory factors promotes survival and growth of spin al cord motoneuron in vitro

Objective To investigate the effects of bone marrow stromal cells (BMSCs) secretory factors on cultured spinal cord motor neuron. Methods BMSCs was cultured from 30 Kunming mice in vitro. BMSCs were purified due to its adherence to plastic. The conditioned media was collected, centrifuged and concentrated at ice cold condition. Then the preparation was added to motor neuron of cultured mouse spinal cord according to different volume percentage concentration. After 24 hours, the surviving activity of motor neurons was examined using MTT method, and the morphology was observed using phase contrast microscope at 72 hours and 1 week. Results The surviving activity of motor neurons was significantly correlated to concentration of the preparation(r = 0.958 9, P < 0.005). And the increase of cell anchoring, diameter, process length, the ratio of cells with process to gross number of cells was paralleled to the rise of concentration of the preparation. Conclusions BMSCs can synthesize and secrete neurotrophic factors physiologically. BMSCs secretory factors play an important role of promoting the survival and growth of motor neuron.