STZ-糖尿病大鼠小肠动力与肠神经胆碱能和氮能神经元关系的研究

目的:探讨链脲佐菌素(STZ)诱导的糖尿病大鼠小肠动力改变与小肠肌间神经丛胆碱能和氮能神经元之间的关系。方法:45只SD大鼠随机分为STZ-糖尿病组、胰岛素组和正常对照组,造模后16周处死。测定大鼠肠动力,采用组织化学染色法观察大鼠小肠肌间神经丛胆碱能和氮能神经元的形态改变。结果:与对照组相比较,糖尿病组和胰岛素组大鼠肠动力均明显减弱(P均<0.05);肌间神经丛胆碱能神经元数目均显著减少(P均<0.01),氮能神经元数量均有减少的趋势(P均>0.05)。胰岛素组肠动力显著高于糖尿病组(P<0.05),但肠神经元计数两组无显著性差异(P>0.05)。结论:STZ-糖尿病大鼠肠动力减弱与肠道肌间神经丛胆碱能和氮能神经损伤有关,胰岛素强化治疗可延缓糖尿病胃肠动力障碍的进展。

Study on the relationship between small intestinal mobility and myenteric plexus cholinergic neuron and nitrergic neuron in STZ-induced diabetic rats

Objective: To study the relationship between intestinal motility and histologic changes of intestinal myenteric plexus cholinergic neuron and nitrergic neuron in STZ-induced diabetic rats. Methods: 45 SD rats were divided into STZ-induced diabetic group, insulin group and control group randomly. The rats were sacrificed at 16 weeks after STZ-induced diabetic model established, intestinal motility was measured and histochemical staining was used to observe histologic changes of intestinal myenteric plexus cholinergic neuron and nitrergic neuron. Results: Compared with control group, both diabetic group and insulin group had obviously slow small intestinal motility (P<0.01, P<0.05), decreased number of intestinal myenteric plexus cholinergic neuron (P<0.01, P<0.01)and nitrergic neuron (P>0.05, P>0.05).Insulin group has significantly rapider small intestinal motility than that of diabetic group (P<0.05), yet the neuron counting had no difference with diabetic group(P>0.05). Conclusion: The abnormal small intestinal mobility in STZ-induced diabetic rats is associated with leisions of the myenteric plexus cholinergic neuron and nitrergic neuron.Insulin intensive therapy can partly ameliorate diabetic gastrointestenal mobility.