大鼠脑挫裂伤半暗带水肿与血-脑屏障破坏的关系

目的研究大鼠局灶性脑挫裂伤半暗带的水肿变化与血-脑屏障(BBB)破坏的关系。方法将126只雄性SD大鼠随机分为3组:假手术组、挫裂伤组和给药组,采用Feeney法制作脑挫裂伤模型,给药组采用曲克芦丁脑蛋白水解物合剂腹腔给药,分别用伊文思蓝(EB)染色法和干湿法观察BBB的变化和脑组织的水肿情况,同时电镜观察超微结构。结果EB染色显示:挫裂伤组和给药组在伤后1 h EB开始漏出,6 h最严重。水含量测定结果显示:挫裂伤组和给药组伤后6 h水含量开始增加,72 h增至高峰。与挫裂伤组比较,给药组脑组织EB溢出量、水含量降低(P<0.05)。结论大鼠脑挫裂伤半暗带BBB通透性改变早于脑水肿的发生,提示BBB破坏可能是早期创伤性脑水肿的结构基础。曲克芦丁脑蛋白水解物合剂可通过改善BBB等多种途径治疗脑挫裂伤。

Relationship of edema and blood-brain barrier disruption in the penumbra area after rat focal brain contusion

Objective To investigate the relationship of edema and blood-brain barrier(BBB) disruption in the penumbra area of rat focal brain contusion.Methods One hundred and twenty-six male SD rats were randomly divided into 3 groups: sham-operation group,contusion group and medication administration group.Brain contusion model was established using Feeney's method.In the medication administration group,the rats were injected intraperitoneally with troxerutin-brain protein hydrolysate mixture.Blood-brain barrier permeability and brain edema were evaluated by Evans blue extravasation and dry-wet weight method,and ultrastructure was observed under electromicroscope.Results In the contusion and medication administration groups,Evans blue extravasation was observed 1 h after trauma,peaked at the 6th hour.In the two groups,the brain water content increased from 6 h after trauma,and peaked at 72 h.Compared with the contusion group,the quantity of Evans blue extravasation and brain water content were lower in the medication administration group(P <0.05).Conclusion After brain contusion,the change in BBB permeability is earlier than brain edema,which suggests that BBB disruption may be the base of early traumatic brain edema.Troxerutin-brain protein hydrolysate mixture is able to inhibit the occurrence and development of traumatic brain edema via improving the blood-brain barrier.