肾纤维囊作为载体培养角膜基质细胞的研究

目的 探索以脱细胞的肾纤维囊作为载体培养角膜基质细胞并研究培养角膜基质细胞特性。方法 将培养扩增的兔角膜基质细胞分别接种到培养板和肾纤维囊上进行体外培养，采用倒置显微镜、免疫组化(波形蛋白)和免疫荧光(AO、PI和Hoechst)的方法进行检测，观察兔角膜基质细胞在培养板和肾纤维囊上的生长情况。结果 倒置显微镜和免疫荧光显微镜观察结果显示角膜基质细胞在肾纤维囊上快速贴壁生长并增殖，细胞形态呈多角形树枝状，多次传代后细胞仍维持原有的形态和功能，细胞能长期培养。在培养板上培养的细胞呈长梭形，细胞贴壁生长和增殖情况稍差。在肾纤维囊上的活细胞较在培养板上培养的细胞多。结论 角膜基质细胞在脱细胞的肾纤维囊载体上生长良好，细胞形态结构明显。本研究为角膜基质细胞的体外培养，提供了简单和高效的方法。

Kidney fiber capsule as a carrier of corneal stromal cell culture

Objective To investigate the feasibility of kidney fiber capsule as carrier for cultivating corneal stromal cells and to study the characteristics of the cultured cells.Methods Rabbit corneal stromal cells were seeded on culture plates or kidney fiber capsule in vitro.Stromal cells were identified by observing live cells under inverted microscope and fluorescent microscope.The cultured cells were stained with vimentin,acridine orange,propidiumiodide and Hoechst.Results Inverted and fluorescent microscope sh...