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| 四氧嘧啶致小鼠糖尿病造模条件优化及稳定性考察 | |
| 引用本文： | 杨明华,杨苏蓓,柴可夫,金祖汉,陈婉姬.四氧嘧啶致小鼠糖尿病造模条件优化及稳定性考察[J].中药药理与临床,2007,23(5):213-216. |
| 作者姓名： | 杨明华 杨苏蓓 柴可夫 金祖汉 陈婉姬 |
| 作者单位： | 1. 浙江省中药研究所,杭州,310023 2. 浙江中医药大学 3. 浙江省立同德医院,杭州,310012 |
| 摘 要： | 目的:优化四氧嘧啶致小鼠糖尿病模型建立的实验条件;评价模型的稳定性。方法:研究小鼠品种、性别、周龄、四氧嘧啶溶媒、给药途径、给药剂量、禁食时间等因素对造模成功率、死亡率及血糖的影响;考察优化后模型的稳定性。结果:小鼠品种、性别、周龄对造模均有影响,是影响造模的主要内在因素;给药途径、给药剂量、禁食时间对造模影响较大,是影响造模的主要外部条件。优化后的模型稳定性较好。结论:选择禁食6小时的ICR小鼠,以尾静脉注射60mg/kg的四氧嘧啶,可以获得成模率高、死亡率较、血糖持续稳定的糖尿病小鼠模型。 |
| 关 键 词： | 四氧嘧啶 小鼠 糖尿病模型 条件优化 |
Optimization of experimental conditions for establishing a diabetes mice model induced by alloxan |
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| Yang Minghua, Yang Subei, Chan Kefu,et al..Optimization of experimental conditions for establishing a diabetes mice model induced by alloxan[J].Pharmacology and Clinics of Chinese Materia Medica,2007,23(5):213-216. | |
| Authors: | Yang Minghua Yang Subei Chan Kefu |
| Institution: | Zhejiang Provincial Research Institute of Chinese Materia Medic , Hangzhou 310023 |
| Abstract: | Objective: To optimize the experimental conditions for establishing a diabetes mice model with alloxan.Methods: These experimental factors,such as species,gender,ages,solution,fasting time,dose and mode delivered by alloxan,et al.have been optimized by evaluating model successful rates,dead rates and blood glucose stability.Results: The results showed that among of all factors,species,fasting time,dose,as well as mode,are main factor.Conclusions: A better diabetes mice model can be gained by tail intravenous injected 60mg/kg alloxan in ICR mice after being fasted 6 hours. |
| Keywords: | diabetes model Optimization of experimental conditions mice alloxan |
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