直肠癌微血管密度与临床病理因素相关性研究

目的本研究回顾性评价直肠癌微血管密度(MVD)与临床病理因素之间的关系。方法采用免疫组化法对87例手术切除直肠癌标本的癌灶中心运用单克隆鼠抗人CD34抗体进行血管标记和染色,然后与临床病理因素分析。结果不同临床病理因素癌灶中心MVD计数表达不同,有无淋巴结转移的MVD计数分别为98.78±26.15、80.12±18.40(P<0.05);浸润深肌层以内及浆膜层及以外分别为83.39±19.70、89.00±28.21(P>0.05);Dukes分期A+B与C+D分别为82.47±18.40、96.50±21.36(P<0.05)。结论直肠癌不同病理因素中MVD表达不同,随着肿瘤分期的进展、淋巴结转移,微血管密度增加。MVD可以作为直肠癌分期、预测直肠癌转移及预后的重要指标。

To Study the Correlation between Microvessel Density and Clinicopathologic Factors in Patients with Rectal Cancer

Objective To investigate the correlation between microvessel density （MVD）and clinicopathologic factors. Methods 87 patients with rectal cancer underwent operative resection. Microvessel density of specimens was evaluated using immunohistochemical staining of surgical specimens with anti - CD34. Results MVD in the Core of cancer were different in different clinicopathologic factors, MVD in positive lymph node metastasis or negative lymph node metastasis were 98.78 ±26.15,80.12 ±18.40（P 〈0.05） respectively. MVD of tumor invasion within muscularis propria or tumor invades through the muscularis propria into the subserosa, into nonperitonealized pericolic or perirectal tissues were 83.39 ± 19.70,89.00 ±28.21 （P 〉 0.05） respectively. MVD in Dukes A ＋ B and Dukes C ＋ D were 82.47 ±18.40,96.50 ±21.36 （ P 〈 0.05 ） respectively. Conclusions MVD was associated with invasive depth, metastasis and disease stage. MVD as a factor to predict the prognosis or metastasis rectal carcinoma, and as a factor to evaluate the stage in rectal carcinoma.