溪黄草袋泡茶的急性毒性和遗传毒性研究

目的： 探讨溪黄草袋泡茶的急性毒性和遗传毒性。方法：急性毒性实验，分别对小鼠灌服剂量为21.5、10.0、4.64、2.15 g/kg的溪黄草袋泡茶提取液，采用霍恩氏(Horn)法计算其半数致死剂量(LD50)。设每皿5 000、1 000、200、40、8 μg共5个剂量组进行Ames试验；分别按 10.0、5.00、2.50 g/kg进行小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验，检测溪黄草袋泡茶的遗传毒性。结果：溪黄草袋泡茶对雌雄小鼠LD50＞21.5 g/kg；Ames试验中，溪黄草袋泡茶对 TA97、TA98、TA100、TA102这4种试验菌株，在加与不加S9条件下，样品各剂量组回变菌落数均未超过溶剂对照组菌落数的2倍，亦无剂量-反应关系；小鼠骨髓嗜多染红细胞微核试验和精子畸形试验结果显示，各剂量组的微核率和精子畸形率与阴性对照组比较差异均无统计学意义(P>0.05)。结论：在本实验条件下，溪黄草袋泡茶属无毒级物质，未见遗传毒性。

Study on the acute toxicity and genetic toxicity of teabags rabdosia serra rabdosia serra

OBJECTIVE： To investigate the acute toxicity and genetic damage in experimental animals. METHODS：In the acute toxicity study,mice were intragastrically administered by 21.5,10.0,4.64,2.15 g/kg rabdosia serra teabags groups and calculated the median lethal dose （LD50） by Horn method.In Ames test,set 5 000, 1 000,200,40,8μg rabdosia serra teabags per plate.In mice bone marrow cell micronucleus and mice sperm abnormality test,2.50,5.00 and 10.0 g/kg rabdosia serra teabags groups.RESULTS：The acute oral toxicity in rabdosia serra teabags was more than 21.5 g/kg.In Ames test with and without S9 metabolic activation,the reverse mutations of typhimurium TA97, TA98,TA100,TA102 were less than double compared with natural mutations,and no dose-response relationship was found.The micronucleus rates and sperm abnormality rates in all doses showed no significant difference from the negative control （P〉0.05）.CONCLUSION：Under these experimental conditions, rabdosia serra teabags was shown to be a non-toxic substance.It did not cause genetic toxicity.