| PI3K/AKT和MAPK/ERK1/2信号通路对胰腺癌PANC-1细胞VEGF表达的影响 |
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| 引用本文： | 张涓娟,蒲宇,李勇,沈成义,张小明.PI3K/AKT和MAPK/ERK1/2信号通路对胰腺癌PANC-1细胞VEGF表达的影响[J].川北医学院学报,2014,29(1):44-48. |
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| 作者姓名： | 张涓娟 蒲宇 李勇 沈成义 张小明 |
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| 作者单位： | 张涓娟 (川北医学院医学影像研究所)； 蒲宇 (川北医学院医学影像研究所)； 李勇 (川北医学院附属医院放射科)； 沈成义 (川北医学院病理生理研究室,四川,南充,637000)； 张小明 (川北医学院医学影像研究所)； |
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| 基金项目： | 国家自然科学基金(项目编号：81271643),川北医学院青年基金(项目编号：CBY11-A-QN01) |
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| 摘 要： | 目的:探讨PI3K/AKT和MAPK/ERK1/2信号通路对人胰腺癌PANC-1血管内皮生长因子(VEGF)表达的影响。方法:抑制剂LY294002和PD98095分别处理人胰腺癌PANC-1细胞,Western blotting检测细胞VEGF蛋白表达。结果:分析表明LY294002和PD98095处理后的PANC-1细胞的磷酸化AKT、磷酸化ERK1/2及VEGF蛋白表达水平均明显低于对照组VEGF(P<0.05)。结论:抑制PI3K/AKT和MAPK/ERK1/2细胞信号通路能够下调VEGF蛋白表达,进而抑制肿瘤新生血管的生成及浸润转移能力。
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| 关 键 词： | 胰腺癌 PI3K/AKT MAPK/ERK1/2 VEGF |
Influence of PI3K and MAPKs signaling pathways on expression of VEGF in human pancreatic cancer PANC-1 cells |
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| ZHANG Juan-juan,PU Yu,LI Yong,SHEN Cheng-yi,ZHANG Xiao-ming.Influence of PI3K and MAPKs signaling pathways on expression of VEGF in human pancreatic cancer PANC-1 cells[J].Journal of North Sichuan Medical College,2014,29(1):44-48. |
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| Authors: | ZHANG Juan-juan PU Yu LI Yong SHEN Cheng-yi ZHANG Xiao-ming |
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| Institution: | 1.Institute of Medical Imaging,North Sichuan Medical College; 2.Department of Radiology,Affiliated Hospital of North Sichuan Medical College; 3.Department of Pathophysiology,North Sichuan Medical College,Nanchong 637000,Sichuan,China;) |
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| Abstract: | Objective：To investigate effects of PI3K/AKT and MAPK/ERK1/2 signaling pathways on human pancreatic cancer PANC-1 cells and expression of vascular endothelial growth factor（VEGF）.Methods：PANC-1 cells,Western blotting testing cells and protein expression of VEGF were exposed to inhibitor LY294002 and PD98059 respectively.Results：The analysis shows that the level of phosphorylation of AKT and ERK in PANC-1 cells and the protein expression of VEGF disposed to inhibitor LY294002 and PD98059 is significantly lower than the control group（P＜0.05）.Conclusion：Inhibiting the PI3K/AKT and MAPK/ERK1/2 signaling pathways can regulate the protein expression of VEGF.Furthermore,it inhibits the production,invasion and metastasis ability of tumor neovascularization. |
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| Keywords: | Pancreatic cancer PI3K/AKT MAPK/ERK1/2 VEGF |
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