睾丸内注射pEGFP-N1体内转染精子并在山羊早期胚胎成功表达

目的考察自制低叶酸饲料对叶酸复合物分子靶向肿瘤程度的影响。方法采用放射性核素标记方法制备^99mTc]DTPA-叶酸。两组不同叶酸含量饲料喂养的荷SGC-7901瘤裸鼠行尾静脉注射^99mTc]DTPA-叶酸，检测4h后组织分布。结果与普通饲料组相比，低叶酸饲料组中^99mTc]DTPA-叶酸在肿瘤组织分布量显著提高，相对增加量达34．5％，其他非靶组织的分布则明显减少。结论喂食自制低叶酸饲料能显著提高DTPA-叶酸对肿瘤组织的靶向程度，为叶酸复合物分子的肿瘤靶向诊断和治疗时选用低叶酸饲料提供了依据。

Transfection of pEGFP - N1 Gene into Goat Spermatozoa in vivo and Its Expression in Early Embryos after Transfection

Objective Intratesticular injection of pEGFP-N 1 was used to investigate its integration in spermatozoa and expression in early embryos . Methods\ Four local male goats were selected and different doses of pEGFP N 1 plasmid DNA were injected into the bilateral testes. The integration of pEGFP N 1 in the sperms was determined by PCR and Southern blotting. Results pEGFP N 1 was integrated into spermatozoa, and the transfection rate peaked 81% at 40 d after intratesticular injection. Expression of green fluorescent protein was found in spermatozoa and early goat embryos with a maximum positive rate of 66 7%. Conclusion\ Intratesticularly injected pEGFP N 1 can be integrated into spermatozoa and then green fluorescent protein can be detected in early embryos produced by in vitro fertilization. Intratesticular injection may be a feasible and simple approach to produce transgenic goats.