载脂蛋白E基因多态性与冠心病的关系分析

目的：研究载脂蛋白E（ApoE)基因多态性与冠心病的关系。方法：选择63例冠心病患者，PCR扩增ApoE基因第4外显子包含编码第112位和第158位氨基酸残基的基因序列，cfoI限制性内切酶酶切后电泳，银染色后分析ApoE基因型。并与年龄、性别相匹配的90例正常对照组比较各基因型及等位基因频率分布。结果：冠心病组E4等位基因频率（12．70％）明显高于对照组（5．55％）（P＜0．05）。结论：E4等位基因可能是冠心病的遗传易患因子。

RELATIONSHIP OF APOLIPOPROTEIN E POLYMORPHISM WITH CORNARY HEART DISEASE

Objective:To detect the relationship of Apolipoprotein E (ApoE) polymorphism with coronary heart disease(CHD).Methods:63 cases of CHD were collected. Exon 4 of Apolipoprotein E fragment coded 112 and 158 amino acid residues was amplified by PCR, and the product was digested by CfoI endonuclease ,after electrophoresis and silver staining,the specific CfoI fragments of different sizes were visualized. And their ApoE alleles frequencies were compared with control subjects(90 cases) whose age, sex were matched with CHD subjects. Results:The frequencies of E 4 allele in patients with CHD(12.70%) were higher than that in control subjects(5.55%)(P<0.05). Conclusion:ApoE 4 allele is probably the genetic marker of CHD.