| 人奶、发、尿样中钙的分光光度测定法 |
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| 引用本文： | 郝俊,赵瑞民,陈雪莲,刘明芝,余世明. 人奶、发、尿样中钙的分光光度测定法[J]. 营养学报, 1986, 0(1) |
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| 作者姓名： | 郝俊 赵瑞民 陈雪莲 刘明芝 余世明 |
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| 作者单位： | 天津医学院，河北遵化县卫生防疫站，内蒙牙克石林业中心防疫站，内蒙牙克石林业中心防疫站，甘肃甘南藏族自治州卫生防疫站 天津 |
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| 摘 要： | 本文报道了以邻-甲酚酞络合剂为显色剂的分光光度法测奶、发、尿中钙。该法灵敏度较高,精密度和回收率好,与等离子体光谱法对比有极好的一致性等特点。使用仪器简单,适用于一般水平的实验室。
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| 关 键 词： | 分光光度法 钙测定 邻-甲酚酞络合剂 |
SPECTROPHOTOMETRIC DETERMINATION OF CALCIUM IN MILK, HAIR AND URINE |
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| Hao Jun,Zhao Ruimin,Chen Xuelian,Liu Mingzhi,and Yushiming. SPECTROPHOTOMETRIC DETERMINATION OF CALCIUM IN MILK, HAIR AND URINE[J]. Acta Nutrimenta Sinica, 1986, 0(1) |
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| Authors: | Hao Jun Zhao Ruimin Chen Xuelian Liu Mingzhi and Yushiming |
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| Abstract: | A spectrophotometric method for the determination of calcium in milk, hair and urine was developed using metal complezing dye orthocresolphth-alein complexone(OCPC).The organic matter in the tested sample was destroyed by digestion. An aliquot of the digested solution was mixed with color developing reagent, alkaline OCPC, cantaining 8-quinolinol to suppress interference such as magnesium, and the absorbance was measured at 570 nm.The coefficients of variation were 0.87, 3.6 and 1.2% for milk, hair and urine samples; and the analytical recoveries of calcium from milk, hair and urine averaged 100.4, 101.0 and 98.5% respectively. The calcium content of milk and hair determined by this method was closely correlated with that of the ICP-AES method (r =0.994, p<0.001 and r= 0.947, p<0.001 respectively). So this method is thought to be convenient for ordinary laboratory. |
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| Keywords: | spectrophotometry calcium determination orthocresolphthalein complexone |
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