| 高效液相色谱法测定吲达帕胺肠溶微球的含量 |
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| 引用本文： | 赵智卉,罗斌华,丁红,范博,阎志飞. 高效液相色谱法测定吲达帕胺肠溶微球的含量[J]. 中国药物与临床, 2010, 10(9): 969-970 |
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| 作者姓名： | 赵智卉 罗斌华 丁红 范博 阎志飞 |
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| 作者单位： | 1. 山西医科大学药学院药剂学教研室,太原,030001
2. 咸宁学院药学院 |
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| 摘 要： | 目的采用反相高效液相色谱法测定吲达帕胺肠溶微球中的含量。方法采用Agilent Eclipse XDB-C18色谱柱(150mm×4.6mm,5μm),以甲醇-水-冰乙酸(45∶55∶0.1)为流动相,检测波长为240nm。结果吲达帕胺在8～48μg/ml与峰面积呈良好的线性关系(r=0.9999),低、中、高浓度的回收率分别为99.55%、98.95%、100.63%,RSD分别为1.15%、0.57%、1.05%。结论所用方法结果准确,专属性强,灵敏度高,重复性好。
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| 关 键 词： | 色谱法 高压液相 吲达帕胺 微球体 |
Determination of indapamide content in enteric microspheres by HPLC |
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| ZHAO Zhi-hui,LUO Bin-hua,DING Hong,FAN Bo,YAN Zhi-fei. Determination of indapamide content in enteric microspheres by HPLC[J]. Chinese Remedies & Clinics, 2010, 10(9): 969-970 |
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| Authors: | ZHAO Zhi-hui LUO Bin-hua DING Hong FAN Bo YAN Zhi-fei |
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| Affiliation: | ZHAO Zhi-hui*,LUO Bin-hua,DING Hong,FAN Bo,YAN Zhi-fei.*Department of Pharmaceutics,College of Pharmacy,Shanxi Medical University,Taiyuan 030001,China |
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| Abstract: | Objective To determine the content of indapamide in enteric microspheres by reversed phase high performance liquid chromatography （RP-HPLC）. Methods Agilent Eclipse XDB-C18 column （150 min×4.6mm, 5 μm） was applied with mobile phase consisting of methanol - water -glacial acetic acid （45：55：0.1） and detection wave-length at 240 nm. Results Indapamide had a good linear relation with peak area within the range of 8-48μg/ml （r= 0.9999）. The recoveries of low, medium and high concentration were 99.55%, 98.95% and 100.63%, with RSD of 1.15%, 0.57% and 1.05% respectively. Conclusion The method used in this study appeared accurate, specific, sensitive and reproducible. |
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| Keywords: | Chromatography high pressure liquid Indapamide Microspheres |
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