| MRL/lpr小鼠中CD4+CD25+调节性T细胞含量、增殖能力及抑制能力的研究 |
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| 引用本文： | 冯林,刁庆春,郑峻松,贺娟,吴军.MRL/lpr小鼠中CD4+CD25+调节性T细胞含量、增殖能力及抑制能力的研究[J].第三军医大学学报,2011,33(4). |
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| 作者姓名： | 冯林 刁庆春 郑峻松 贺娟 吴军 |
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| 作者单位： | 重庆市第一人民医院皮肤科;第三军医大学医学检验系临床检验学教研室;第三军医大学西南医院全军烧伤研究所创伤、烧伤与复合伤国家重点实验室; |
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| 基金项目： | 重庆市自然科学基金重点项目(07-1-023)~~ |
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| 摘 要： | 目的探讨MRL/lpr小鼠中CD4+CD25+调节性T细胞(Treg细胞)的含量、增殖能力及其对效应性T淋巴细胞(Teff细胞)的抑制能力及意义。方法流式细胞检测法检测8只MRL/lpr小鼠及8只BALB/c小鼠脾脏中Treg细胞的水平;磁珠分选法分选出脾脏中的Treg细胞与Teff细胞,离体培养2周后,分别测量Treg细胞数量,并比较差异;用Treg细胞与Teff细胞组成共培养体系,5 d后检测Teff细胞的水平,计算Treg细胞对Teff细胞的抑制率并比较差异。结果 MRL/lpr小鼠脾脏中Treg细胞的总含量与BALB/c小鼠无统计学差异(4.88±0.55)%vs(5.03±0.34)%,P>0.05];离体培养2周后MRL/lpr小鼠的Treg细胞数量显著低于BALB/c小鼠(6.61±0.92)×105vs(13.25±1.91)×105,P<0.05];共培养5 d后MRL/lpr小鼠Treg细胞对Teff细胞的抑制率显著低于BALB/c小鼠Treg细胞(P<0.05)。结论 MRL/lpr小鼠体内Treg细胞增殖能力、抑制能力的下降可能导致体内免疫平衡的破坏、自身抗原-抗体反应,参与MRL...
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| 关 键 词： | 调节性T细胞 增殖能力 抑制能力 MRL/lpr小鼠 |
Population, proliferative ability and suppressive activity of CD4+CD25+ regulatory T cells in MRL/lpr mice |
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| Feng Lin,Diao Qingchun,Zheng Junsong,He Juan,Wu Jun.Population, proliferative ability and suppressive activity of CD4+CD25+ regulatory T cells in MRL/lpr mice[J].Acta Academiae Medicinae Militaris Tertiae,2011,33(4). |
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| Authors: | Feng Lin Diao Qingchun Zheng Junsong He Juan Wu Jun |
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| Institution: | Feng Lin1,Diao Qingchun1,Zheng Junsong2,He Juan2,Wu Jun3(1Department of Dermatology,Chongqing First People's Hospital,Chongqing,400010,2Department of Clinical Laboratory Sciences,College of Medical Laboratory,3State Key Laboratory of Trauma,Burns and Combined Injury,Institute of Burns,Southwest Hospital,Third Military Medical University,400038,China) |
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| Abstract: | Objective To investigate the percentage,reproductive ability of CD4+CD25+ regulatory T cells(Tregs) in MRL/lpr mice,and their suppressive activity to effective T cells(Teffs).Methods Flow cytometry was used to determine cell populations of Tregs in the spleen of 8 MRL/lpr mice and 8 BALB/c mice respectively.Magnetic beads were employed to separate Tregs and Teffs from the splenic cells of the 2 kinds of mice.Then the Tregs were cultured in vitro for 2 weeks and cell populations were determined.The Tregs wer... |
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| Keywords: | regulatory T cell MRL/lpr mice reproductive activity suppressive activity |
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