| 盐酸氨溴索对体外白色假丝酵母菌成熟生物膜的抑制作用及其形态学的影响 |
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| 引用本文： | 吴玉华,陆彪,余加林. 盐酸氨溴索对体外白色假丝酵母菌成熟生物膜的抑制作用及其形态学的影响[J]. 第三军医大学学报, 2011, 33(4) |
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| 作者姓名： | 吴玉华 陆彪 余加林 |
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| 作者单位： | 宁夏医科大学附属医院儿童急救科;宁夏医科大学附属医院儿科;重庆医科大学附属儿童医院新生儿科儿童发育疾病研究省部共建教育部重点实验室; |
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| 基金项目： | 国家自然科学基金(30772363,81070513); 2007年度高等学校博士学科点专项科研基金(20070631001); 2008重庆医科大学校级重点项目(XBZD200808)~~ |
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| 摘 要： | 目的研究盐酸氨溴索对体外白色假丝酵母菌(Candida albicans)成熟生物膜(biofilm,BF)的影响。方法用微孔板法建立体外白色假丝酵母菌ATCC 90028 BF模型;采用甲基四氮盐(the abated tetrazolium salt,XTT)减低法定量评价盐酸氨溴索对白色假丝酵母菌成熟BF的抑制作用;银染后,倒置显微镜下观察该药对白色假丝酵母菌成熟BF的形态学影响。结果在96孔微量细胞培养板上成功建立白色假丝酵母菌BF模型;1.25、2.5、5、7.5 mg/ml的盐酸氨溴索作用白色假丝酵母菌成熟BF 12 h后,XTT减低法D(450)值分别为(0.63±0.05)、(0.52±0.08)、(0.31±0.05)和(0.11±0.03),分别与空白对照组(0.71±0.07)比较,差异有显著性(P<0.05);0.625 mg/ml的盐酸氨溴索作用白色假丝酵母菌成熟BF后,D(450)值为(0.67±0.04),与空白对照组比较,差异无显著性(P>0.05);不同浓度的盐酸氨溴索作用白色假丝酵母菌成熟BF,组间比较,均有显著性差异(P<0.05)。结论盐酸氨溴索对体外白色假丝酵母菌成熟...
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| 关 键 词： | 白色假丝酵母菌 生物膜 盐酸氨溴索 |
Inhibition of ambroxol hydrochloride on Candida albicans biofilm in vitro and its effect on morphogenesis |
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| Wu Yuhua,Lu Biao,Yu Jialin. Inhibition of ambroxol hydrochloride on Candida albicans biofilm in vitro and its effect on morphogenesis[J]. Acta Academiae Medicinae Militaris Tertiae, 2011, 33(4) |
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| Authors: | Wu Yuhua Lu Biao Yu Jialin |
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| Affiliation: | Wu Yuhua1,Lu Biao2,Yu Jialin3(1Department of Children's Emergency,2Department of Pediatrics,Affiliated Hospital,Ningxia Medical University,Yinchuan,Ningxia Hui Autonomous Region,750004,3Department of Neonatology,Key Laboratory of Developmental Diseases in Childhood of Ministry of Education,Children's Hospital,Chongqing Medical University,Chongqing,400014,China) |
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| Abstract: | Objective To investigate the effect of ambroxol hydrochloride on biofilm and morphology of Candida albicans(C.albicans) in vitro.Methods Microtiter plate culture method was used to establish C.albicans(ATCC 90028) biofilm.The abated tetrazolium salt(XTT) reduction assay was performed to quantitatively analyze the effect of ambroxol hydrochloride against C.albicans mature biofilm for 12 h.Microscopic examination with silver staining was conducted to assess the effect of ambroxol hydrochloride on morpho-logy ... |
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| Keywords: | Candida albicans biofilm ambroxol hydrochloride |
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